{"product_id":"abcam-ab173539","title":"Abcam, ab173539, Anti-Raf1 (phospho S259) antibody [EPR3433(2)]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nAnti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) is a rabbit monoclonal antibody detecting Raf1 in  Western Blot, Dot Blot . Suitable for  Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR3433(2),\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:WB, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Dot Blot in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of Raf1?\u003cbr\u003e\nAnti-Raf1 (phospho S259) [EPR3433(2)] (ab173539) specifically detects a band for Raf1 (UniProt: P04049) at a molecular weight of 73kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-Raf1 (phospho S259) [EPR3433(2)] (ab173539) was first used in a scientific publication in 2013 and has been cited over 10 times in peer-reviewed journals.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR3433(2)] also available for your convenience: ab173539, Carrier free -\u003cbr\u003e\nab240957\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRaf1 also known as c-Raf or Raf-1 is a serine\/threonine-protein kinase that plays an important role in cell division differentiation and survival. Its molecular weight is approximately 74 kDa. Raf1 is part of the MAPK\/ERK signaling pathway and is ubiquitously expressed in many tissues including the brain heart and liver. As a component of the signaling cascade its activation translates extracellular signals into intracellular responses.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRaf1 regulates important cellular processes by activating downstream kinases in response to external stimuli. Raf1 forms a complex with other proteins such as Ras facilitating its role as an essential component of the MAPK\/ERK pathway. Upon activation by Ras Raf1 phosphorylates and activates MEK1 and MEK2 which in turn activate the ERK1 and ERK2. This signaling axis is involved in controlling gene expression and cellular proliferation.\u003cbr\u003e\nPathways\u003cbr\u003e\nRaf1 is integrally involved in the MAPK\/ERK pathway which is critical for transducing signals from growth factors and mitogens. It relates closely with proteins such as Ras MEK and ERK in this pathway. The pathway is important for regulating cellular responses to various stimuli and is particularly involved in processes such as cell cycle control and apoptosis.\u003cbr\u003e\nRaf1 has associations with certain types of cancer and Noonan syndrome. In various cancers mutations or dysregulation of Raf1 or related components such as Ras can lead to uncontrolled cellular proliferation. In Noonan syndrome Raf1 mutations result in anomalies in the Ras\/MAPK pathway which can impact normal development. Raf1's interactions in these pathways indicate its relevance as a potential target for therapeutic intervention.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850239299753,"sku":"ab173539","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab173539","provider":"Iright","version":"1.0","type":"link"}