{"product_id":"abcam-ab176609","title":"Abcam, ab176609, Anti-ATP6AP1\/ATP6S1 antibody","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRabbit Polyclonal ATP6AP1\/ATP6S1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6AP1 aa 1-250.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Recombinant Fragment Protein within Human ATP6AP1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.Q15904\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Lyophilized, Reconstitution-reconstitute with water at 200µL, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nATP6AP1 also known as ATPase H+ Transporting Accessory Protein 1 is a component of the V-ATPase complex. This protein weighing about 55 kDa assists in the acidification of organelles such as endosomes and lysosomes. ATP6AP1 exists mainly in the endoplasmic reticulum and is also found in the Golgi apparatus. It interacts with other proteins to maintain proper pH levels in intracellular compartments which is important for various cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nATP6AP1 acts as a regulatory subunit of the vacuolar ATPase (V-ATPase) proton pump complex. This complex is responsible for pumping protons across intracellular and plasma membranes which acidifies vacuoles and lysosomal compartments. Such acidification is necessary for degradation processes within lysosomes. Its association with V-ATPase shows that ATP6AP1 influences pH balance rather than enzyme activity playing a role in cellular homeostasis and trafficking.\u003cbr\u003e\nPathways\u003cbr\u003e\nATP6AP1 functions within both the acidification and autophagy pathways. Maintaining the acidic environment needed for endocytic and autophagic pathways ensures proper degradation and recycling of cellular components. ATP6AP1 associates with proteins like ATP6V1A an important component of the V-ATPase linking it to proton translocation processes. The protein’s activity within these pathways supports vesicle acidification needed for processes like protein degradation and cellular waste elimination.\u003cbr\u003e\nATP6AP1 mutations have connections to glycosylation disorders and congenital disorders of glycosylation type IIH. Alterations in the gene encoding ATP6AP1 can lead to improper protein glycosylation and trafficking within the cell. This malfunction is associated with symptoms that affect multiple systems and cause developmental issues. Additionally ATP6AP1 has an established relationship with another protein ATP6V0A2 through pathways linked to glycosylation demonstrating its importance in maintaining cellular function and health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46854383829161,"sku":"ab176609","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab176609","provider":"Iright","version":"1.0","type":"link"}