{"product_id":"abcam-ab176663","title":"Abcam, ab176663, NF kappaB p65 (pS536 + Total) ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nNF kappaB p65 (pS536 + Total) ELISA Kit is a single-wash 90-min Simplestep used to detect NF kappaB p65 (pS536 + Total). The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Semi-quantitative Sandwich ELISA - 450 nm readout : works on any standard plate reader - Cited in over 25 citations\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue Homogenate, Cell Lysate,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nAssay type:Semi-quantitative,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's NFkB p65 (pS536) and NFkB p65 (Total)\u003cbr\u003e\nin vitro\u003cbr\u003e\nSimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of NFkB p65 (pS536) and Total NFkB p65 protein in Human and mouse cells.\u003cbr\u003e\nThe SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody\/analyte\/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.\u003cbr\u003e\nAs of October 2019, this kit was reformulated with new antibodies to maintain continued long term supply.\u003cbr\u003e\nEstimated sensitivity\u003cbr\u003e\n: Phospho-NF-κB p65 (Ser536): 5 μg\/mL (tested with HeLa), Total NF-κB p65: 5 μg\/mL (tested with HeLa)\u003cbr\u003e\n: Phospho-NF-κB p65 (Ser536): 6-600 μg\/mL, Total NF-κB p65: 6-600 μg\/mL\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNF-kB p65 also known as RelA is a transcription factor with a molecular mass of around 65 kDa. It is a vital component of the NF-kB family typically found in the cytoplasm in its inactive form. Upon activation it translocates to the nucleus where it regulates the expression of target genes. NF-kB p65 is ubiquitously expressed in various cell types including immune cells and epithelial cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNF-kB p65 plays a central role in regulating immune response and inflammation. It forms a complex with proteins such as p50 to bind DNA and modulate gene transcription. This complex is essential for cellular responses to stress and infection influencing processes such as cell proliferation and survival. It also interacts with other transcription factors and cofactors to fine-tune gene regulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nNF-kB p65 is integral to the canonical NF-kB signaling pathway which gets activated by signals like cytokines and bacterial products. The protein family includes several related proteins such as IκB that inhibit NF-kB activity until signal-induced degradation allows NF-kB to enter the nucleus. Another related pathway is the MAPK pathway which can interact with NF-kB signaling to coordinate cellular stress responses.\u003cbr\u003e\nNF-kB p65 is implicated in conditions such as cancer and inflammatory diseases. Its dysregulation can lead to chronic inflammation contributing to tumor progression. Inflammatory disorders like rheumatoid arthritis also show abnormal NF-kB activity contributing to disease pathology. Additionally interactions with proteins like IKK contribute significantly to these diseases altering NF-kB signaling and gene expression patterns.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843609809065,"sku":"ab176663","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab176663","provider":"Iright","version":"1.0","type":"link"}