{"product_id":"abcam-ab180880","title":"Abcam, ab180880, Hela-DFO treated (0.5mM, 24h) Nuclear Lysate","description":"\u003cp\u003eSize: 200µg\u003cbr\u003e\nHela-DFO treated (0.5mM, 24h) Nuclear Lysate. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe recommend aliquoting the extracts into single use fractions and then storing them at -80°C.\u003cbr\u003e\nThe cells were treated for 24 hours with a 0.5mM solution of Deferoxamine (DFO). DFO is used as a hypoxia-mimetic agent to stabilize Hypoxia Inducible Factor 1 (HIF-1). DFO stabilizes HIF-1 through the inhibition of Prolyl Hydroxylases (PHDs) which target HIF-1 through degradation. The mechanism of DFO inhibition is likely through the chelation of Fe2+ bound to the active site of PHD which is required for enzymatic activity.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845182640297,"sku":"ab180880","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab180880","provider":"Iright","version":"1.0","type":"link"}