{"product_id":"abcam-ab181616","title":"Abcam, ab181616, Anti-Rb antibody [EPR17512]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-Rb antibody [EPR17512] (ab181616) is a rabbit monoclonal antibody detecting Rb in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC\/IF . Suitable for  African green monkey, Human, Mouse . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 80 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR17512,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Human, African green monkey,\u003cbr\u003e\nApplications:IHC-P, WB, IP, Flow Cyt (Intra), ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nProduct Specifications\u003cbr\u003e\nAnti-Rb antibody [EPR17512] (ab181616) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC\/IF, IHC-P, IP, WB in african green monkey, human, mouse samples.\u003cbr\u003e\nAnti-Rb antibody [EPR17512] (ab181616) specifically detects Rb (UniProt ID: P13405; Molecular weight: 105kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).\u003cbr\u003e\nQuality and Validation\u003cbr\u003e\nAbcam's high quality manufacturing and validation processes ensure Anti-Rb antibody [EPR17512] (ab181616) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.\u003cbr\u003e\nThe specificity of Anti-Rb antibody [EPR17512] (ab181616) has been confirmed by testing in knockout samples.\u003cbr\u003e\nAnti-Rb antibody [EPR17512] (ab181616) has been cited over 83 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nRelated Products\u003cbr\u003e\nAntibody clone EPR17512 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 594, Alexa Fluor® 488, Alexa Fluor® 555 (\u003cbr\u003e\nab300158\u003cbr\u003e\nab300670\u003cbr\u003e\nab300671\u003cbr\u003e\nab300672\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRetinoblastoma protein (Rb) also known as pRb is an important regulatory protein with a molecular weight of approximately 110 kDa. It is mainly expressed in the nucleus of cells. Rb functions as a tumor suppressor by controlling the cell cycle progression from G1 to S phase. Rb becomes active when it is dephosphorylated allowing it to bind and inhibit E2F transcription factors consequently preventing the transcription of genes essential for S phase entry.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRetinoblastoma protein influences cellular proliferation differentiation and apoptosis. It acts within a larger protein complex modulating various cellular responses. When functional Rb halts uncontrolled cell division important for maintaining normal tissue homeostasis. In its phosphorylated state known as phospho-Rb or phospho-Rb E182 it loses its regulatory capabilities which can lead to unrestrained cell cycle progression.\u003cbr\u003e\nPathways\u003cbr\u003e\nSeveral involve the retinoblastoma protein. One key pathway is the p53 pathway which Rb interacts with to influence cellular outcomes like cell-cycle arrest and apoptosis. Rb cooperates with proteins like p21 to implement these processes. Additionally it is involved in the cyclin D-dependent kinase 4 (CDK4) pathway which modulates its phosphorylation state influencing binding interactions and cell cycle control.\u003cbr\u003e\nDysregulation of retinoblastoma protein is commonly associated with cancer particularly retinoblastoma and breast cancer. In retinoblastoma mutations in the Rb gene directly lead to uncontrolled cell proliferation due to the absence of functional Rb protein. Additionally Rb's connection to the E2F family of transcription factors can become disrupted contributing to oncogenesis in other cancers.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855754055849,"sku":"ab181616","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab181616","provider":"Iright","version":"1.0","type":"link"}