{"product_id":"abcam-ab186028","title":"Abcam, ab186028, Cellular ROS Assay Kit (Orange)","description":"\u003cp\u003eSize: 200Test\u003cbr\u003e\nCellular ROS Assay Kit (Orange) ab186028 uses an ROS sensor to quantify ROS in live cells.\u003cbr\u003e\nKey facts\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay Platform:Microplate reader, Fluor. microscope, Flow cyt.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCellular ROS Assay Kit (Orange) ab186028 uses an ROS sensor to quantify ROS in live cells. The orange dye used in the ROS assay protocol is cell-permeable and generates orange fluorescence when it reacts with ROS. The fluorescence signal of the dye can be measured by fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry.\u003cbr\u003e\nab186028 provides a sensitive fluorometric, one-step assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour. The ROS assay can be performed in a convenient 96-well or 384-well microtiter-plate format using either a fluorescence microplate reader at Ex\/Em = 540\/570 nm or a fluorescent microscope with TRITC filter.\u003cbr\u003e\nPreviously called Cellular Reactive Oxygen Species Detection Assay Kit (Orange Fluorescence).Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways.**Related products**Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.To measure reactive oxygen species within cells, we recommend . Alternative ROS assays are available in orange (ab186028), red (\u003cbr\u003e\nab186027\u003cbr\u003e\n), and deep red (\u003cbr\u003e\nab186029\u003cbr\u003e\nab238535\u003cbr\u003e\nis used to measure ROS in biofluids, culture supernatants and cell lysates. For assays designed to differentiate ROS, superoxides, and reactive nitrogen species: to assay ROS and superoxides use\u003cbr\u003e\nab139476\u003cbr\u003e\n; to assay ROS, superoxides, and reactive nitrogen species use\u003cbr\u003e\nab139473\u003cbr\u003e\n; to assay superoxides use\u003cbr\u003e\nab219943\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843642478761,"sku":"ab186028","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab186028","provider":"Iright","version":"1.0","type":"link"}