{"product_id":"abcam-ab196434","title":"Abcam, ab196434, Recombinant human PRMT6 protein","description":"\u003cp\u003eSize: 20µg\u003cbr\u003e\nRecombinant human PRMT6 protein is a Human Full Length protein, in the 2 to 375 aa range, expressed in Escherichia coli, with \u0026gt;45%, suitable for SDS-PAGE, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;45% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:His tag N-Terminus,\u003cbr\u003e\nApplications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Specific Activity 0.015 pmol\/min\/ÂμgAssay Conditions: 50 μl reaction mix (20 mM phosphate buffer pH 7.4, 20 μM S-adenosylmethionine, and 1-5 ng methyltransferase PRMT6) is added to microwells coated with histone substrate. Incubate at 30°C for 2 hr. Add antibody against methylated Arg residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.,\u003cbr\u003e\nAccession:Q96LA8,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 8Preservative: 1.36% ImidazoleConstituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPRMT6 or Protein Arginine Methyltransferase 6 is an enzyme that adds methyl groups to arginine residues on target proteins. It is a member of the PRMT family and has a molecular mass of approximately 42 kDa. This enzyme is widely expressed in nucleous where it modifies histones and various nuclear proteins influencing chromatin structure and gene expression.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPRMT6 plays a pivotal role in regulating transcription by methylating histone H3 at arginine 2 (H3R2) which suppresses gene expression. It forms part of larger chromatin remodeling complexes that modulate access to DNA. PRMT6 also interacts with p53 an important regulator of the cell cycle impacting its transcriptional activity and stability.\u003cbr\u003e\nPathways\u003cbr\u003e\nPRMT6 is central to epigenetic and transcriptional regulation pathways. It functions alongside proteins like p53 and Mdm2 within the DNA damage response pathway where it influences cell proliferation and repair processes. This enzyme further relates to the Akt signaling pathway which affects cell growth and survival although it is not the primary pathway.\u003cbr\u003e\nPRMT6 is implicated in cancer and systemic lupus erythematosus (SLE). In cancer deregulation of PRMT6 can lead to uncontrolled cell growth by affecting genes related to cell cycle and proliferation often interacting with protein p53. In systemic lupus erythematosus altered PRMT6 activity may contribute to abnormal immune responses associating with proteins involved in autoimmunity.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843899478185,"sku":"ab196434","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab196434","provider":"Iright","version":"1.0","type":"link"}