{"product_id":"abcam-ab204537","title":"Abcam, ab204537, Creatinine Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 2 x 96Tests\u003cbr\u003e\nCreatinine Assay Kit (Colorimetric) (ab204537) is a complete kit for the quantitative determination of creatinine in urine. Creatinine Assay Kit (Colorimetric) designed for success: - Fast homogeneous mix-and-ready assay with no wash steps, results in just 30 minutes - Calibrated against the NIST standard and offers reproducible results with less than 6% inter- and intra-assay variation. - Highly sensitive, of which the detection limit can be as low as 0.042 mg\/dL. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Urine,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nAssay time:30m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCreatinine Assay Kit (Colorimetric) (ab204537) is a complete kit for the quantitative determination of creatinine in urine.\u003cbr\u003e\nCreatine assay method\u003cbr\u003e\nCreatinine Assay Kit ab204537 is based upon the Jaffe reaction. Creatinine reacts with alkaline picrate to produce an orange color. The color intensity is measured before and after adding acid, and the difference is proportional to the creatinine concentration.\u003cbr\u003e\nCreatinine Assay protocol summary:\u003cbr\u003e\n- Add samples to wells\u003cbr\u003e\n- Add reaction mix and incubate for 30 minutes at room temperature\u003cbr\u003e\n- Measure optical density (OD490 nm)\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nSee below other alternative Creatinine Assay Kits:\u003cbr\u003e\n- Creatinine Assay Kit\u003cbr\u003e\nab65340\u003cbr\u003e\n- Albumin Creatinine Ratio Assay Kit\u003cbr\u003e\nab241018\u003cbr\u003e\n- Protein Creatinine Ratio Assay Kit\u003cbr\u003e\nab272539\u003cbr\u003e\nOther Notes\u003cbr\u003e\nCreatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or is synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and p-creatine are converted nonenzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine, and under normal conditions, its formation occurs at a relatively constant rate. Intra-individual variation of creatinine levels is \u0026lt;15% from day to day, making it a useful marker for normalizing levels of other molecules found in urine. Altered creatinine levels may be associated with conditions that result in decreased renal blood flow, such as diabetes and cardiovascular disease.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843616723113,"sku":"ab204537","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab204537","provider":"Iright","version":"1.0","type":"link"}