{"product_id":"abcam-ab205811","title":"Abcam, ab205811, GSH\/GSSG Ratio Detection Assay Kit II (Fluorometric - Green)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nGSH\/GSSG Ratio Detection Assay Kit II ab205811 is used to quantify total glutathione, GSH and GSSG. Simply add probe(s) and incubate at room temperature for 10-60 mins. Readout on any fluorometric (Ex\/Em = 490\/520 nm) plate reader. - Cited in \u0026gt;60 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Urine, Plasma, Tissue Extracts, Cell Lysate,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:= 10 nM,\u003cbr\u003e\nAssay time:30m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThe GSH\/GSSG assay protocol uses a proprietary non-fluorescent water-soluble dye that becomes strongly fluorescent upon reacting with GSH. With a one-step fluorimetric method, the kit can detect as little as 1 picomole of GSH or GSSG in a 100 µL assay volume.\u003cbr\u003e\nThe assay can be performed in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation without a separation step. Its signal can be easily read by a fluorescence microplate reader at Ex\/Em = 490\/520 nm.\u003cbr\u003e\nGSH\/GSSG assay protocol summary:\u003cbr\u003e\n- add samples (deproteinized) and standards to wells\u003cbr\u003e\n- for GSH assay add Thiol Green in assay buffer, or for total glutathione (GSH + GSSG) assay also add GSSG probe\u003cbr\u003e\n- incubate for 10 - 60 min at room temp\u003cbr\u003e\n- analyze with microplate reader\u003cbr\u003e\nGSSG levels can be calculated by subtracting GSH from total glutathione levels.\u003cbr\u003e\nNOTE: For measuring GSH Standard only, there is enough reagent provided to perform 200 tests.\u003cbr\u003e\nHow other researchers are using\u003cbr\u003e\nGSH\/GSSG Ratio Detection Assay Kit II (Fluorometric - Green) has been used in a variety of sample type including:\u003cbr\u003e\n- Human umbilical cord mesenchymal stem cells (hUCMSCs) 1\u003cbr\u003e\n- Mouse kidney tissues 2\u003cbr\u003e\n- Rat Brain homogenates 3\u003cbr\u003e\nReferences:\u003cbr\u003e\n1 - Hu B et al. 2023; 2 - Gong Q et al. 2023; 3 - Fuchs M et al. 2023.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843600502953,"sku":"ab205811","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab205811","provider":"Iright","version":"1.0","type":"link"}