{"product_id":"abcam-ab207616","title":"Abcam, ab207616, Mouse APE ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse APE ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse APE with a sensitivity of 28.8 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture extracts, Tissue Extracts, Cell culture supernatant,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 28.8 pg\/mL,\u003cbr\u003e\nRange:46.88 - 3000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse APE ELISA Kit (ab207616) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of APE protein in cell culture extracts, cell culture supernatant, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse APE with 28.8 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n- Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n- High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n- Fully validated in biological samples\u003cbr\u003e\n- 96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nMouse APE is a multifunctional protein that binds DNA and RNA and plays a central role in the cellular response to oxidative stress. The two major activities of APE are DNA repair and redox regulation of transcription factors. APE functions as an apurinic\/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. The C-terminal region of APE is responsible for base excision repair activities. Notably, this repair activity is suppressed by phosphorylation. The N-terminal region of APE may regulate the DNA binding capabilities of a variety of transcription factors through redox stimulation. Mouse APE has 98% and 94% identity to rat and human APE, respectively.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAPE1 also known as APEX1 or apurinic\/apyrimidinic endonuclease 1 functions as an important DNA repair enzyme. It plays a pivotal role in the base excision repair (BER) pathway where it recognizes and processes apurinic\/apyrimidinic sites in DNA. APE1 has a molecular mass of about 37 kDa. It is expressed primarily in the nucleus with detectable levels in the cytoplasm. The expression of APE1 spans across various tissue types indicating its essential role in maintaining genomic stability.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAPE1 enzymatically cleaves the phosphodiester bond at abasic sites creating a nick in the DNA backbone for further repair steps. This action prevents mutations and maintains DNA integrity. APE1 also functions as a redox factor regulating the transcriptional activity of several transcription factors. It is not part of a larger complex but interacts with various BER pathway proteins such as DNA polymerase beta and XRCC1. This interaction is important for the effective repair of damaged DNA and cellular response to oxidative stress.\u003cbr\u003e\nPathways\u003cbr\u003e\nAPE1 is integrated within the base excision repair and redox signaling pathways. These pathways are fundamental for repairing single-strand breaks and modulating the cellular oxidative stress response. APE1 coordinates closely with other proteins like PNKP and OGG1 in the BER pathway ensuring precise and effective DNA repair. Through its redox activity APE1 influences pathways involving NF-kB and AP-1 demonstrating its multifaceted roles in cellular processes.\u003cbr\u003e\nAlterations in APE1 function associate with cancer progression and neurodegenerative diseases. Overexpression or mutations in APE1 correlate with increased tumor resistance to chemotherapy in several cancers including lung and ovarian cancer. APE1's interaction with proteins like p53 and HMGB1 connects it to the etiology and progression of these malignancies. Furthermore impaired APE1 activity links to neurodegenerative disorders such as Alzheimer's disease where DNA repair deficiency contributes to neuronal damage and cognitive decline.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843583004841,"sku":"ab207616","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab207616","provider":"Iright","version":"1.0","type":"link"}