{"product_id":"abcam-ab211163","title":"Abcam, ab211163, Cdc42 Activation Assay Kit","description":"\u003cp\u003eSize: 20Test\u003cbr\u003e\nCdc42 Activation Assay Kit (ab211163) uses PAK1 PBD Agarose beads to selectively isolate and pull-down the active form of Cdc42 from purified samples or endogenous lysates.\u003cbr\u003e\nKey facts\u003cbr\u003e\nSample types:Tissue, Suspension cells, Adherent cells,\u003cbr\u003e\nReacts with:Mouse, Rat, Human\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCdc42 Activation Assay Kit (ab211163) uses PAK1 PBD Agarose beads to selectively isolate and pull-down the active form of Cdc42 from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Cdc42 is detected by western blot analysis using an anti-Cdc42 specific monoclonal antibody, which reacts with the human, mouse and rat protein.\u003cbr\u003e\nFeatures: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis\/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.\u003cbr\u003e\nSmall GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Cdc42, a 21 kDa protein, belongs to the family of Rho GTPases and regulates a variety of biological response pathways that include cell motility, cell division, gene transcription, and cell transformation. Like other small GTPases, Cdc42 regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Cdc42 binds specifically to the p21-binding domain (PBD) of p21-activated protein kinase (PAK) to control downstream signaling cascades.\u003cbr\u003e\nCdc42 is overexpressed in NSCLC (non-small cell lung cancer), colorectal adenocarcinoma, melanoma, breast cancer and testicular cancer.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843639824553,"sku":"ab211163","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab211163","provider":"Iright","version":"1.0","type":"link"}