{"product_id":"abcam-ab211925","title":"Abcam, ab211925, Anti-Alas1 antibody [EPR10247] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Alas1 antibody. Carrier free. Suitable for WB, ICC\/IF and reacts with Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR10247,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab211925 is the carrier-free version of\u003cbr\u003e\nab154860\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\n'Alas1' also known as 5-Aminolevulinic acid synthase 1 is a mitochondrial enzyme with a mass of approximately 64 kDa. This enzyme plays an important role in heme biosynthesis catalyzing the first step in the pathway by converting glycine and succinyl-CoA to 5-aminolevulinic acid. Unfortunately it does not function alone and often requires the presence of pyridoxal phosphate as a cofactor. 'Alas1' is ubiquitously expressed in a variety of tissues including liver and bone marrow where heme production is critical.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\n'Alas1' serves as a rate-limiting enzyme in the heme synthesis pathway. Being the first step it sets the pace for the entire process. It does not form part of any larger protein complex and operates with its required cofactors. Beyond metabolic roles its regulation influences several cellular processes ensuring heme availability precisely matches cellular demand.\u003cbr\u003e\nPathways\u003cbr\u003e\n'Alas1' performs a fundamental role in the mitochondrial heme biosynthesis pathway. This pathway is essential for synthesizing heme a component critical to various cellular functions such as oxygen transport and electron transfer. 'Alas1' interacts with proteins like ferrochelatase the enzyme completing the heme biosynthesis pathway. Coordination between 'Alas1' and other proteins ensures efficient production of heme enabling proper cellular function and adaptation to changes in cellular and systemic conditions.\u003cbr\u003e\n'Alas1' is involved in conditions such as acute intermittent porphyria and certain anemias. Its dysregulation can lead to impaired heme production with downstream effects on cell metabolism and organ function. 'Alas1' has been shown to interact with HMBS (hydroxymethylbilane synthase) a protein linked to different types of porphyria. Understanding its role in these conditions could lead to targeted therapies that modulate its activity to alleviate symptoms or correct underlying biochemical dysfunctions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851865313449,"sku":"ab211925","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab211925","provider":"Iright","version":"1.0","type":"link"}