{"product_id":"abcam-ab213472","title":"Abcam, ab213472, Human IRAK4 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 1 x 384Tests\u003cbr\u003e\nHuman IRAK4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IRAK4 with a sensitivity of 247.4 pg\/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 384-well plate for higher throughput - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture extracts, Tissue Extracts,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 247.4 pg\/mL,\u003cbr\u003e\nRange:0.5 - 32 ng\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (6 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman IRAK4 ELISA Kit ab213472 is a rapid single-wash 90-min Sandwich ELISA to measure Human IRAK4 in cell culture extracts, tissue extracts. This SimpleStep sensitivity is 247.4 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman IRAK4 SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman IRAK4 ELISA Kit ab213472 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nSave your precious samples\u003cbr\u003e\nOur 384-well Human IRAK4 ELISA Kit only requires a maximum of 12.5µL sample\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-IRAK4 antibody [EPR19671-185] - BSA and Azide free (Capture)\u003cbr\u003e\nab242833\u003cbr\u003e\n- Anti-IRAK4 antibody [EPR19671-170] - BSA and Azide free (Detector)\u003cbr\u003e\nab242833\u003cbr\u003e\nIRAK4 is a serine\/threonine protein kinase involved in the innate immune response. IRAK4 is recruited by MD88 through TLR signalling, where it phosphorylates IRAK1 and ultimately results in NF-kappa-B nuclear translocation and activation.The standard is affinity purified.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nIRAK4 also known as Interleukin-1 receptor-associated kinase 4 is an important protein with an approximate molecular mass of 51 kDa. This protein is a significant part of the signaling cascade initiated by the Interleukin-1 receptor (IL-1R) and Toll-like receptors (TLRs). Expressed in a variety of tissues including the spleen thymus and lung IRAK4 is involved in innate immune responses. The presence of IRAK4 protein facilitates the downstream signaling pathways that activate the transcription factors responsible for inflammatory cytokine production.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nIRAK4 plays an important role in mediating signaling in the innate immune system by forming complexes with other proteins such as IRAK-1. These complexes propagate signals that result in the activation of transcription factors like NF-kB and AP-1 which regulate the expression of inflammatory genes. Through these mechanisms IRAK4 influences the body's ability to respond to infections and stresses. Its critical involvement in these signaling pathways highlights its importance in maintaining immune homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nIRAK4 is a pivotal player in the Toll-like receptor and IL-1 receptor signaling pathways which are central to the host defense against pathogens. It functions upstream activating the MyD88-dependent pathway resulting in the recruitment and phosphorylation of other kinases such as IRAK-1 and TRAF6. These interactions trigger the NF-kB signaling cascade thereby enhancing pro-inflammatory cytokine production that is essential for initiating immune responses.\u003cbr\u003e\nMutations or dysregulation of IRAK4 are linked to increased susceptibility to recurrent bacterial infections and sepsis. Patients with IRAK4 deficiency have impaired NF-kB activation leading to a weakened inflammatory response. Additionally overexpression of IRAK4 has been observed in certain autoimmune diseases such as rheumatoid arthritis where the persistent activation of TLR signaling contributes to chronic inflammation. The role of IRAK4 in these disorders ties closely with its interaction with proteins like TRAF6 influencing disease development and progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843578024105,"sku":"ab213472","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab213472","provider":"Iright","version":"1.0","type":"link"}