{"product_id":"abcam-ab213653","title":"Abcam, ab213653, Anti-SLC12A1\/NKCC2 antibody [EPR11842] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal SLC12A1\/NKCC2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR11842,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:The homology between SLC12A1\/NKCC2 and SLC12A2 is 67%, so ab213653 may cross react with SLC12A2. We have not performed any laboratory testing to validate this.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab213653 is the carrier-free version of\u003cbr\u003e\nab171747\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe SLC12A1 protein also known as the NKCC2 transporter is an important player in ion transport within renal physiology. With a molecular weight of around 120 kDa NKCC2 functions in the reabsorption of sodium potassium and chloride ions a function facilitated by its location in the thick ascending limb of the loop of Henle in the kidneys. This location and functionality are critical for renal concentration mechanisms and contribute to the maintenance of electrolyte and fluid balance.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis transporter plays an essential role in concentrating urine and regulating blood pressure. NKCC2 is a part of the larger solute carrier family and does not usually form complexes with other proteins. It facilitates the trans-epithelial movement of Na+ K+ and 2Cl- ions from the lumen into renal epithelial cells which is fundamental for the creation of an osmotic gradient essential for water reabsorption.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe SLC12A1 protein is a significant component of renal electrolyte transport and blood pressure regulation pathways. It is intricately involved in the renin-angiotensin-aldosterone system (RAAS) which regulates blood volume and systemic vascular resistance. In these pathways NKCC2's activity tightly connects it to other ion channel proteins and transporters such as ENaC which also play roles in blood pressure regulation by influencing sodium handling in the nephron.\u003cbr\u003e\nDysregulation of NKCC2 function can result in Bartter syndrome and hypertension. Bartter syndrome a rare disorder characterized by salt wasting and electrolyte imbalances stems from mutations affecting NKCC2. This transporter also impacts hypertension where its dysregulation can alter sodium reabsorption processes linking it to other proteins involved in blood pressure control such as the mineralocorticoid receptor. Understanding the role of NKCC2 provides insight into potential therapeutic targets for these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851910205609,"sku":"ab213653","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab213653","provider":"Iright","version":"1.0","type":"link"}