{"product_id":"abcam-ab213796","title":"Abcam, ab213796, Human IL-1 RL1 ELISA Kit (ST2)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman IL-1 RL1 ELISA Kit (ST2) is a Sandwich (quantitative) ELISA for the measurement of Human IL-1 RL1 (ST2) in Human in Cell\/Tissue Extracts, Cell Culture Media, Biofluids samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 10 pg\/mL,\u003cbr\u003e\nRange:156 - 10000 pg\/mL,\u003cbr\u003e\nAssay time:3h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThe Human IL-1 RL1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ST2) (ab213796) is designed for the quantitative detection of Human IL1RL1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).\u003cbr\u003e\nThe ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for IL-1 RL1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: K19-S328) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 RL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL1RL1amount of sample captured in plate.\u003cbr\u003e\nInterleukin 1 receptor-like 1, also known as IL-1 RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by pro-inflammatory stimuli and may be involved in the function of helper T cells. TL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I (IL-1 RL1), interleukin 1 receptor, type II (IL-1 R2) and interleukin 1 receptor like 2 (IL-1 RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nST2 also known as IL-1RL1 or IL-1RL1 chimera protein is a member of the IL-1 receptor family. It has a molecular mass of approximately 55 kDa. This protein exists in different forms including a membrane-bound form and a soluble variant. Researchers typically find ST2 expressed in various tissues including the lungs gut and cardiovascular system. The expression level can vary significantly depending on the physiological or pathological state.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.\u003cbr\u003e\nPathways\u003cbr\u003e\nIL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33\/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.\u003cbr\u003e\nST2 has significant associations with asthma and cardiovascular diseases. In asthma the IL-33\/ST2 axis critically contributes to airway inflammation and hyperresponsiveness. Similarly in the context of cardiovascular diseases elevated levels of soluble ST2 are markers of heart failure with the ST2\/IL-33 axis contributing to cardiac remodeling. ST2's connection with inflammatory processes implies it also interacts with other cytokines such as interleukin-6 (IL-6) exacerbating the disease states through shared pathways.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855675281577,"sku":"ab213796","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab213796","provider":"Iright","version":"1.0","type":"link"}