{"product_id":"abcam-ab214275","title":"Abcam, ab214275, Anti-ErbB2 \/ HER2 antibody [EPR19547-12]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nAnti-ErbB2 \/ HER2 antibody [EPR19547-12] (ab214275) is a rabbit monoclonal antibody detecting ErbB2 \/ HER2 in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC\/IF, ELISA . Suitable for  Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR19547-12,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:I-ELISA, Flow Cyt (Intra), IHC-P, IP, ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:Only reacts with weakly in mouse 4T1 cells.This antibody could recognize ErbB2 and weak cross reaction with ErbB4. Mouse and rat species are recommended based on WB results, we do not guarantee IHC-P for mouse and rat.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-ErbB2 \/ HER2 antibody [EPR19547-12] (ab214275) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF), ELISA in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of ErbB2 \/ HER2?\u003cbr\u003e\nAnti-ErbB2 \/ HER2 [EPR19547-12] (ab214275) specifically detects a band for ErbB2 \/ HER2 (UniProt: P04626) at a molecular weight of 137kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-ErbB2 \/ HER2 [EPR19547-12] (ab214275) was first used in a scientific publication in 2016 and has been cited over 10 times in peer-reviewed journals.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR19547-12] also available for your convenience: ab214275, Carrier free -\u003cbr\u003e\nab222482\u003cbr\u003e\n, PE -\u003cbr\u003e\nab225508\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab225509\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab225510\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.\u003cbr\u003e\nPathways\u003cbr\u003e\nErbB2 plays a pivotal role in pathways such as the PI3K\/Akt and MAPK\/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K\/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK\/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.\u003cbr\u003e\nErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844077801641,"sku":"ab214275","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab214275","provider":"Iright","version":"1.0","type":"link"}