{"product_id":"abcam-ab214663","title":"Abcam, ab214663, Annexin V-CF Blue 7-AAD Apoptosis Staining \/ Detection Kit","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nAnnexin V-CF Blue 7-AAD Apoptosis Staining \/ Detection Kit (ab214663) contains Annexin V labeled with CF Blue, which allows the identification and quantitation of apoptotic cells on a single-cell basis by flow cytometry.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Cell-based (quantitative),\u003cbr\u003e\nAssay Platform:Flow cytometer\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnnexin V-CF Blue 7-AAD Apoptosis Staining \/ Detection Kit (ab214663) contains Annexin V labeled with CF Blue, which allows the identification and quantitation of apoptotic cells on a single-cell basis by flow cytometry. Simultaneous staining of cells with Annexin V–CF Blue (blue fluorescence) and the non-vital dye 7-aminoactinomycin D (7-AAD) (red fluorescence) allows the discrimination of intact cells (Annexin V-CF Blue negative, 7-AAD Staining Solution negative), early apoptotic (Annexin V-CF Blue positive, 7-AAD Staining Solution negative) and late apoptotic or necrotic cells (Annexin V-CF Blue positive, 7-AAD Staining Solution positive).\u003cbr\u003e\nCF-Blue can be used as direct replacement for Pacific Blue™ or similar blue dyes.\u003cbr\u003e\nFor more apoptosis assays, review the full set of\u003cbr\u003e\nAnnexin V assays\u003cbr\u003e\n, or the\u003cbr\u003e\napoptosis assay and apoptosis marker guide\u003cbr\u003e\nExposure of phosphatidylserine (PS) on the external surface of the cell membrane has been reported to occur in the early phases of apoptotic cell death, during which the cell membrane remains intact. In leukocyte apoptosis, PS on the outer surface of the cell marks the cell for recognition and phagocytosis by macrophages. The Annexin V protein has a high affinity for PS, and shows minimal binding to phosphatidylcholine and sphingomyelin. Changes in PS asymmetry, which can be analyzed by measuring annexin V binding to the cell membrane, are generally observed before morphological changes associated with apoptosis occurred and before membrane integrity is lost.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843620819113,"sku":"ab214663","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab214663","provider":"Iright","version":"1.0","type":"link"}