{"product_id":"abcam-ab218260","title":"Abcam, ab218260, Oligonucleotide Conjugation Kit","description":"\u003cp\u003eSize: 1 x 100µg \/ 3 x 100µg\u003cbr\u003e\nAbcam's Oligonucleotide Conjugation Kit (ab218260) offers several standout features: - Rapid Conjugation: enables generation of antibody-oligonucleotide conjugates in less than 2 hours. - High Efficiency: achieves high levels of antibody and oligonucleotide recovery, with conjugation efficiency ~ 95-100%. - Versatility: Suitable for conjugating antibodies to single-stranded oligonucleotides (10-120 bases) and double-stranded oligonucleotides (up to 80 bases) - Positive control included\u003cbr\u003e\nKey facts\u003cbr\u003e\nAssay time:2h\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThe Oligonucleotide Conjugation Kit (ab218260) enables the easy and efficient generation of antibody-oligonucleotide conjugates in less than 2 hours. The kit can be used to generate conjugates of different ratios of antibody: oligonucleotide. It also includes positive controls that allow the user to confirm that the conjugation chemistry has worked correctly.\u003cbr\u003e\nThis kit can be used to conjugate antibodies to single-stranded oligonucleotides that are 10-120 bases long or to double-stranded oligonucleotides that are up to 80 bases long. The oligonucleotide should be synthesized to include a terminal amine group.\u003cbr\u003e\nThe 1 x 100 μg kit includes reagents for 1 conjugation of 100 μg of antibody and 1 control.\u003cbr\u003e\nThe 3 x 100 μg kit includes reagents for 3 conjugations of 100 μg of antibody and 1 control.\u003cbr\u003e\nAs expected for any chemical conjugation, the concentration and buffer formulation of the oligonucleotide and the antibody need to fall within certain parameters, as detailed in the protocol booklet.\u003cbr\u003e\nBenefits\u003cbr\u003e\nQuick and easy to use – Save time, no specialist knowledge required\u003cbr\u003e\nHigh levels of antibody and oligo recovery – Conjugation efficiency is about 95-100%.\u003cbr\u003e\nUse your own oligo and antibody, at your desired ratio – Flexible\u003cbr\u003e\nFreeze dried – Ships at ambient temperature, long shelf life\u003cbr\u003e\nStringently QC tested – Consistent high quality, excellent batch to batch reproducibility\u003cbr\u003e\nUnidirectional chemistry – No risk of crosslinking\u003cbr\u003e\nCovalent bond – Highly stable conjugates\u003cbr\u003e\nSuitable for single stranded oligos of 10–120 bases, and double stranded oligos up to 80 base pairs – Covers the majority of sequences\u003cbr\u003e\nLinking chemistry works at both 5' and 3' end – Provides ability to combine with other modifications\u003cbr\u003e\nPost-conjugation clean-up step – No interference from unbound oligo's\u003cbr\u003e\nPositive control antibody and oligo included – Enables confirmation of protocol success\u003cbr\u003e\nA wide range of target proteins – Also applicable to antibody fragments and small proteins.\u003cbr\u003e\nThis product is manufactured by Expedeon, an Abcam company, and was previously called Thunder-Link\u003cbr\u003e\nPlus Oligo Conjugation Kit. 425-0300 is the same as the 3 x 100 μg size. 425-0000 is the same as the 1 x 100 μg size.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847598100649,"sku":"ab218260","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab218260","provider":"Iright","version":"1.0","type":"link"}