{"product_id":"abcam-ab219915","title":"Abcam, ab219915, Caspase-3\/7, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nCaspase-3\/7, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric) ab219915 is used to monitor multiple caspase activation in live cells - 3 fluorogenic indicators for simultaneous monitoring of caspase3\/7, caspase 8 and caspase 9 - Optimised for microplate reader - Up to 100 tests for each caspase\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Semi-quantitative,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCaspase-3\/7, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric) is designed to simultaneously monitor three key caspases involved in apoptosis: the initiator caspases caspase 8 and caspase 9, and the executioner Caspase 3.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThe kit uses DEVD-ProRed™, IETD-R110 and LEHD-AMC as fluorogenic indicators for Caspase 3, caspase 8 and caspase 9 activity respectively. Upon caspase cleavage, three distinct fluorophores are released: ProRed™ (red fluorescence), R110 (green fluorescence) and AMC (blue fluorescence), which can be readily monitored in a single assay due to their spectral separation.\u003cbr\u003e\nThis product has been optimized for use in a microplate reader in a 96-well plate, providing enough reagent to perform 100 tests for each caspase.\u003cbr\u003e\nCaspase-3\/7, Caspase-8 and Caspase-9 protocol summary\u003cbr\u003e\n- Grow and treat cells with test compounds to induce caspase activation\u003cbr\u003e\n- Add caspase assay solution to cells\u003cbr\u003e\n- Incubate at room temperature for 30-60 minutes\u003cbr\u003e\n- Monitor fluorescence intensity at: Ex\/Em = 535\/620 nm (Caspase 3\/7), Ex\/Em = 490\/525 nm (Caspase 8), Ex\/Em = 370\/450 nm (Caspase 9)\u003cbr\u003e\nHow other researchers are using this kit\u003cbr\u003e\nab219915 has been used in a variety of cancer cell lines including MCF-7 (breast cancer) cell line (1), U87MG (human glioblastoma) and MES-SA (human uterine sarcoma) cell lines (2).\u003cbr\u003e\nReferences: 1 - T. Kumkoon et al. 2023; 2 - Yu Hsia et al. 2023.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843611480233,"sku":"ab219915","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab219915","provider":"Iright","version":"1.0","type":"link"}