{"product_id":"abcam-ab22035","title":"Abcam, ab22035, Anti-Nestin antibody [10C2] - Neural Stem Cell Marker","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035) is a mouse monoclonal antibody detecting Nestin in  Western Blot . Suitable for  Human . - Over 150 publications - Trusted since 2005\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:10C2,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Recombinant Fragment Protein within Human NES aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P48681\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.\u003cbr\u003e\nWhat is the molecular weight of Nestin?\u003cbr\u003e\nAnti-Nestin [10C2] - Neural Stem Cell Marker (ab22035) specifically detects a band for Nestin (UniProt: P48681) at a molecular weight of 176kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-Nestin [10C2] - Neural Stem Cell Marker (ab22035) was first used in a scientific publication in 2005 and has been cited over 150 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-Nestin [10C2] - Neural Stem Cell Marker (ab22035) has over 5 independent reviews from customers.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNestin also known as NES is an intermediate filament protein with a molecular weight of about 220 kDa. Nestin features prominently in cells related to the nervous system including neural stem cells and it serves as a structural component in these cells. Nestin also appears in substantial amounts during the development of the nervous system and is important for cell structural organization. It is often used as a Nestin marker in research particularly with Nestin antibodies for identifying the presence of neural stem cells and tracking their developmental course. Researchers frequently utilize techniques like Nestin IHC (immunohistochemistry) and Nestin western blot to stain and detect Nestin allowing detailed investigations into its expression in various tissues.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNestin plays a significant role in the dynamic remodeling of the cytoskeleton. It does not act as a solitary element but forms part of a larger protein complex involved in maintaining and organizing cellular structure. This property grants cells flexibility in shape and resilience during the rapid changes in development and specialization. The role of Nestin in the cellular architecture allows it to contribute to processes such as cell migration and proliferation critical during tissue development and repair.\u003cbr\u003e\nPathways\u003cbr\u003e\nNestin integrates into networks that regulate stem cell behavior and neurogenesis. It tightly interacts within the pathways involving cell cycle regulation and differentiation. For example the Wnt and Notch signaling pathways modulate its expression to influence neural stem cell fate and patterning during development. Protein interactions between Nestin and other cytoskeletal elements like vimentin facilitate its roles in these pathways adapting the cellular environment for specific developmental activities.\u003cbr\u003e\nNestin associates with various pathological conditions including brain tumors and muscular dystrophies. Its expression is aberrant in many forms of gliomas where it often signifies a higher grade of malignancy. Within these tumors Nestin connects to oncogenic signaling pathways involving proteins such as the epidermal growth factor receptor (EGFR). Moreover in muscular dystrophies Nestin's expression in regenerating muscle fibers illustrates its involvement in cellular repair mechanisms potentially interacting with dystrophin a protein important for muscle integrity. Research on anti-Nestin antibodies continues to clarify its role in these diseases potentially offering diagnostic or therapeutic insights.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848264994985,"sku":"ab22035","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab22035","provider":"Iright","version":"1.0","type":"link"}