{"product_id":"abcam-ab227900","title":"Abcam, ab227900, Human BAG3 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman BAG3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BAG3 with a sensitivity of 11.7 pg\/well. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture extracts, Tissue Extracts,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 11.7 pg\/well,\u003cbr\u003e\nRange:39.1 - 2500 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman BAG3 ELISA Kit ab227900 is a rapid single-wash 90-min Sandwich ELISA to measure Human BAG3 in cell culture extracts, tissue extracts. This SimpleStep sensitivity is 11.7 pg\/weLL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman BAG3 SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman BAG3 ELISA Kit ab227900 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-Bag3 antibody [EPR20207-4] - BSA and Azide free (Capture)\u003cbr\u003e\nab244958\u003cbr\u003e\n- Anti-Bag3 antibody [EPR20207-109] - BSA and Azide free (Detector)\u003cbr\u003e\nab244958\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Bcl-2-associated athanogene 3 commonly known as Bag3 is a protein with a molecular weight of approximately 74 kDa. It plays an important role in apoptosis regulation and stress responses by interacting with heat shock proteins. Bag3 is expressed in various tissues including the heart skeletal muscles and certain types of neurons. It functions mechanically by binding to chaperones like Hsp70 modulating their activity and helping in protein folding and degradation processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nBag3 contributes to cell survival by assisting in protein homeostasis and mitigating stress-related cellular damage. It acts as a co-chaperone and forms part of complexes with Hsp70 HspB8 and CHIP among others. These interactions enhance the cellular capacity to handle misfolded proteins preventing the aggregation that could lead to cell toxicity. In muscle cells Bag3 plays a role in maintaining structural integrity by participating in the assembly of myofibrils critical for muscle function.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe protein is involved in autophagy and apoptosis regulation. It actively participates in the ubiquitin-proteasome system and chaperone-mediated autophagy collaborating closely with proteins like HspB8 and Hsp70. The integration of Bag3 within these pathways highlights its involvement in removing damaged proteins and preserving cellular function during stress conditions. Additionally Bag3 contributes to the regulation of the NF-kB pathway which is involved in the immune response and inflammation.\u003cbr\u003e\nMutations or dysregulation of Bag3 associate with conditions such as myopathy and certain cancers. In myopathy aberrations in Bag3 affect muscle resilience and repair leading to structural muscle defects. In cancer Bag3's role in inhibiting apoptosis promotes tumor cell survival making it a target for therapeutic interventions. The protein has connections with p53 in cancer contexts where it influences cell death and survival decisions. Understanding Bag3's interactions and functions in these diseases opens new opportunities for developing treatments targeting these pathways.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843591983273,"sku":"ab227900","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab227900","provider":"Iright","version":"1.0","type":"link"}