{"product_id":"abcam-ab228556","title":"Abcam, ab228556, Calcein AM Assay Kit (Fluorometric)","description":"\u003cp\u003eSize: 1000Test\u003cbr\u003e\nCalcein AM Assay Kit (ab228556) is a sensitive quantitative assay to measure the cell viability of adherent and suspension cells. - Fluorescent readout - use with microplate reader - Detect as low as 50 viable cells in less than 30 min\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Cell-based (quantitative),\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCalcein AM Assay Kit (ab228556) can detect as low as 50 viable cells in less than 30 minutes.\u003cbr\u003e\nHow the Calcein AM assay works\u003cbr\u003e\nCalcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells. During the Calcein AM assay, hydrolysis of Calcein AM by intracellular esterases produces a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm and can be measured at Ex\/Em = 485\/530 nm. The measured fluorescence intensity is proportional to the number of viable cells.\u003cbr\u003e\nThe Calcein AM assay can be used for cell proliferation, cell viability, and cytotoxicity studies.\u003cbr\u003e\nCalcein AM assay protocol summary\u003cbr\u003e\n- prepare cells\u003cbr\u003e\n- prepare Cell Dye II\/Calcein AM solution\u003cbr\u003e\n- add prepared Cell Dye II\/Calcein AM solution to each well\u003cbr\u003e\n- incubate at 37ºC for 30 minutes\u003cbr\u003e\n- read fluorescence (Ex\/Em = 485\/530 nm)\u003cbr\u003e\nReview the to learn about more kits to perform a , and .\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843645984937,"sku":"ab228556","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab228556","provider":"Iright","version":"1.0","type":"link"}