{"product_id":"abcam-ab228562","title":"Abcam, ab228562, Beta Galactosidase Assay Kit (Senescence)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nBeta Galactosidase Assay Kit (Senescence) ab228562 is used to fluorescently detect senescence-associated Beta Galactosidase activity in cell cultures by flow cytometry. Senescent cells are stained green. Readout in FITC channel. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Cell-based (quantitative),\u003cbr\u003e\nAssay Platform:Flow cytometer\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nSenescence Assay Kit (Beta Galactosidase, Fluorescence) (ab228562) is designed to fluorescently detect senescence-associated Beta Galactosidase activity in cultured cells by flow cytometry. The senescence-associated Beta Galactosidase is present only in senescent cells and is not found in pre-senescent, quiescent or immortal cells.\u003cbr\u003e\nOther Beta Galactosidase Assay Kits and Staining Kits\u003cbr\u003e\nTo measure beta galactosidase activity as part of a senescence assay:\u003cbr\u003e\n- use Beta Galactosidase Staining Kit (Senescence)\u003cbr\u003e\nab65351\u003cbr\u003e\nto stain senescent cells blue and visualize by light microscopy\u003cbr\u003e\nTo measure beta galactosidase activity as part of a LacZ assay with cell cultures:\u003cbr\u003e\n- use Beta Galactosidase Assay Kit (LacZ, Green)\u003cbr\u003e\nab176721\u003cbr\u003e\nin an easy-to-use assay with readout on fluorometric plate reader\u003cbr\u003e\n- use Beta Galactosidase Assay Kit (LacZ, Blue)\u003cbr\u003e\nab189815\u003cbr\u003e\nwith readout on fluorometric plate reader or flow cytometer\u003cbr\u003e\n- use Beta Galactosidase Staining Kit (LacZ)\u003cbr\u003e\nab102534\u003cbr\u003e\nto stain LacZ cells blue and visualize by light microscopy\u003cbr\u003e\nBeta Galactosidase Assay Kit (Green)\u003cbr\u003e\nab287846\u003cbr\u003e\nis used to measure Beta Galactosidase in homogenized cell or tissue samples with readout on fluorometric plate reader.\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product was previously called K991 Biovision Senescence Detection Kit (Fluorometric). Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSenescence-associated beta-galactosidase often called SA-beta-gal is an enzyme that serves as a biomarker for cellular senescence. It is a lysosomal hydrolase with a molecular mass of approximately 120 kDa. This enzyme functions mechanically by catalyzing the hydrolysis of beta-galactosides into monosaccharides. SA-beta-gal is expressed extensively in senescent cells which accumulate in various tissues as organisms age.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSenescence-associated beta-galactosidase functions as an indicator of the senescent state of cells. It does not act as part of a larger complex but is key in detecting an increase in lysosomal mass characteristic of senescence. During the aging process cells exhibit increased expression of this enzyme which scientists use in beta-galactosidase assays to identify senescent cells in culture. Fluorescence methods or beta-gal staining often facilitate visualization of senescent cells by binding to substrates processed by the enzyme.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe enzyme is indicative of involvement in both the p53 and p16INK4a-Rb tumor suppression pathways. Although not an active participant itself SA-beta-gal reflects altered cellular metabolism linked to these pathways often in collaboration with proteins like p53 and p16INK4a which regulate cell cycle arrest. Additionally it aligns with the decline of mitochondrial function another hallmark of senescent cells influencing cellular aging and stress response pathways.\u003cbr\u003e\nSA-beta-gal activity correlates strongly with age-related diseases such as Alzheimer's disease and certain types of cancer. As an aged cell marker its elevated presence can signify increased cellular senescence often contributing to tissue dysfunction and promoting disease progression. In Alzheimer's disease connections to amyloid precursor protein processing and tau pathology have been observed. In cancer SA-beta-gal works alongside proteins like p53 and p16INK4a to highlight cell cycle arrest anomalies where downregulated functionality or bypass can lead to unchecked cellular proliferation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843618394281,"sku":"ab228562","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab228562","provider":"Iright","version":"1.0","type":"link"}