{"product_id":"abcam-ab229408","title":"Abcam, ab229408, Human TPA ELISA Kit, Fluorescent","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman TPA ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human TPA with a sensitivity of 7.27 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Fluorescent Sandwich ELISA - 530\/570\/590 nm readout : works on any standard plate reader\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 7.27 pg\/mL,\u003cbr\u003e\nRange:9.77 - 20000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nTissue Plasminogen Activator (TPA)\u003cbr\u003e\nin vitro\u003cbr\u003e\nCatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Plasminogen Activator (TPA) protein in human serum, plasma and cell culture supernatant.\u003cbr\u003e\nThis CatchPoint SimpleStep ELISA kit has been\u003cbr\u003e\noptimized for Molecular Devices Microplate Readers\u003cbr\u003e\n. Click\u003cbr\u003e\nfor a list of recommended Microplate Readers.\u003cbr\u003e\nIf using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at\u003cbr\u003e\nwww.softmaxpro.org\u003cbr\u003e\nThe CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody\/analyte\/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530\/570\/590 nm Excitation\/Cutoff\/Emission.\u003cbr\u003e\nTissue Plasminogen Activator (Tissue-type plasminogen activator or TPA) is a circulating serine protease involved in the breakdown of clots. TPA converts inactive plasminogen to active plasmin; in turn plasmin degrades the fibrin matrix in clots. In addition, plasmin can cleave TPA at Arg-310 with results in a two chain disulphide linked TPA that has even greater proteolytic activity. TPA is synthesized in many tissues and is secreted into most extracellular body fluids. Recombinant TPA is used medically to resolve or prevent blood clots in ischemic stroke or myocardial infarction.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTissue Plasminogen Activator (tPA) also known as a plasminogen activator acts mechanically to convert plasminogen to plasmin a serine protease. This reaction occurs at the surface of a fibrin clot leading to clot degradation a process known as fibrinolysis. tPA has a molecular mass of approximately 70 kDa. It is mainly expressed in vascular endothelial cells and is released into the bloodstream in response to stimuli such as circulatory stasis or endothelial damage.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTPA plays a critical role in thrombolysis by breaking down blood clots into their soluble components. It regulates plasminogen function by cleaving this zymogen to yield the active protease plasmin. This function makes tPA integral in maintaining hemostasis and it does not form a part of a larger protein complex. The activity and the regulation of tPA are important for preventing pathologic clotting which can lead to cardiovascular complications.\u003cbr\u003e\nPathways\u003cbr\u003e\nTPA is central to the fibrinolytic pathway. This pathway facilitates the conversion of plasminogen to plasmin enabling clot resolution. In addition tPA interacts with other proteins such as urokinase plasminogen activator (uPA) and inhibitors like plasminogen activator inhibitor-1 (PAI-1). The balance between tPA and its inhibitors is important for the regulation of fibrinolytic activity impacting hemostatic and thrombotic events.\u003cbr\u003e\nTPA connects closely with conditions like stroke and myocardial infarction due to its thrombolytic properties. In ischemic stroke excessive or insufficient tPA activity can disrupt normal blood flow leading to tissue damage. Additionally in myocardial infarction tPA's role in breaking down clots proves important for restoring coronary blood flow. It is also linked with proteins like fibrinogen as they serve as substrates in the clot degradation process and with PAI-1 which modulates its activity and influences disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855636877481,"sku":"ab229408","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab229408","provider":"Iright","version":"1.0","type":"link"}