{"product_id":"abcam-ab229891","title":"Abcam, ab229891, Rat Leptin ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nRat Leptin ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Leptin with a sensitivity of 24 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Rat,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 24 pg\/mL,\u003cbr\u003e\nRange:47 - 3000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nRat Leptin ELISA Kit (ab229891) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Leptin protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Leptin with 24 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n- Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n- High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n- Fully validated in biological samples\u003cbr\u003e\n- 96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nLeptin also known as the OB protein is a hormone with a molecular weight of approximately 16 kDa. Primarily it is produced by adipocytes in white adipose tissue and to a lesser extent in the placenta stomach and other tissues. This protein plays a significant role in energy homeostasis by conveying information to the central nervous system regarding fat storage. In scientific studies leptin is often measured using methods like the leptin ELISA kit or mouse leptin assay to monitor its levels and function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nLeptin regulates food intake and energy expenditure by binding to its receptors in the hypothalamus. It is an important part of the feedback loop to manage body weight and appetite. Leptin is not part of a larger protein complex and acts directly on its target tissues. By signal transduction pathways it influences cellular processes such as metabolism and even impacts fertility and immune responses.\u003cbr\u003e\nPathways\u003cbr\u003e\nLeptin is involved in the neuroendocrine signaling pathway and the MAPK\/ERK pathway. In the neuroendocrine system leptin interacts with proteins like proopiomelanocortin (POMC) and neuropeptide Y (NPY) to manage appetite and energy use. Additionally it activates the JAK\/STAT pathway when binding to its receptor which influences gene expression involved in metabolism and inflammation.\u003cbr\u003e\nLeptin is linked to obesity and type 2 diabetes. Leptin deficiencies or resistance can lead to metabolic disorders as the hormone's signaling is disrupted affecting energy balance and storage. Its connection to adiponectin another hormone derived from fat tissue is critical in understanding these conditions; while leptin promotes inflammation adiponectin has anti-inflammatory effects. Exploring these proteins' roles and their balance offers insights into treatments for obesity-related disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843653390505,"sku":"ab229891","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab229891","provider":"Iright","version":"1.0","type":"link"}