{"product_id":"abcam-ab233471","title":"Abcam, ab233471, Lipid Peroxidation (MDA) Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 200Test\u003cbr\u003e\nLipid Peroxidation (MDA) Assay Kit (Colorimetric) ab233471 enables researchers to quantify MDA without the heating steps required by the TBARS assay conventionally used for MDA detection. Readout on any colorimetric (695 nm) plate reader.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue Lysate, Suspension cells, Adherent cells,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nIn this Lipid Peroxidation (MDA) Assay, the MDA Color Reagent reacts with MDA to generate a blue color product which is measured at 695 nm with absorbance microplate readers.\u003cbr\u003e\nLipid peroxidation is characterized by the oxidative degradation of unsaturated fatty acids, phospholipids, glycolipids, cholesterol esters and cholesterol. Malondialdehyde (MDA) is one of the most commonly used biomarkers for lipid peroxidation.\u003cbr\u003e\nRunning an MDA assay has historically relied on a reaction with thiobarbituric acid (the TBARS assay) to generate a product that can be measured colorimetrically at 532 nm or fluorimetrically at Ex\/Em = 530\/550 nm.\u003cbr\u003e\nHowever, the TBARS assay has quite a few limitations:\u003cbr\u003e\n- the reaction is not specific to MDA,\u003cbr\u003e\n- the TBA-MDA reaction needs be run under acidic conditions,\u003cbr\u003e\n- the TBARS assay needs be run under high temperature, commonly at 90-100 °C.\u003cbr\u003e\nLipid Peroxidation (MDA) Assay Kit (Colorimetric) protocol summary:\u003cbr\u003e\n- add samples and standards to wells\u003cbr\u003e\n- add MDA color reagent and incubate for 10-30 min at room temp\u003cbr\u003e\n- add reaction solution and incubate for 30-60 min at room temp\u003cbr\u003e\n- analyze with microplate reader\u003cbr\u003e\nHow other researchers are using\u003cbr\u003e\nLipid Peroxidation (MDA) Assay Kit (Colorimetric) has been used in a variety of sample type including:\u003cbr\u003e\n- Human GC cell lines include SNU719, AGS, MKN45, and HGC-27 1\u003cbr\u003e\n- Human breast cancer MDA-MB-468 2\u003cbr\u003e\n- Mouse Kidney tissues 3\u003cbr\u003e\nReferences:\u003cbr\u003e\n1 - Wang J et al. 2024; 2 -Alsrhani A et al. 2023; 3 - Pham T et al. 2023.\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nSee an alternative kit, TBARS assay kit for MDA measurement:\u003cbr\u003e\n- Lipid Peroxidation (MDA) Assay Kit (Colorimetric\/Fluorometric)\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843609383081,"sku":"ab233471","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab233471","provider":"Iright","version":"1.0","type":"link"}