{"product_id":"abcam-ab233613","title":"Abcam, ab233613, Human Sorbitol Dehydrogenase ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Sorbitol Dehydrogenase ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Sorbitol Dehydrogenase with a sensitivity of 60 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Urine, Cell culture extracts, Tissue Extracts, Citrate plasma, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 60 pg\/mL,\u003cbr\u003e\nRange:0.39 - 25 ng\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Sorbitol Dehydrogenase ELISA Kit (ab233613) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Sorbitol Dehydrogenase protein in cit plasma, serum, tissue extracts, urine, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Sorbitol Dehydrogenase with 60 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n- Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n- High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n- Fully validated in biological samples\u003cbr\u003e\n- 96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSorbitol Dehydrogenase (SDH) also known as SORD is an enzyme that catalyzes the oxidation of sorbitol to fructose. This reaction involves the conversion of a secondary alcohol group to a ketone. SDH has a molecular mass of approximately 38 kDa. It is expressed in various human tissues most importantly in the liver kidneys and seminal vesicles. The enzyme requires NAD+ as a cofactor for its catalytic activity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSDH plays a part in the polyol pathway which is important for the metabolism of glucose into fructose. It functions alongside aldose reductase to regulate the conversion of glucose into sorbitol and then sorbitol into fructose. SDH acts independently without forming a complex with other proteins. The amount and activity of SDH can affect cellular osmotic balance and energy storage.\u003cbr\u003e\nPathways\u003cbr\u003e\nSDH is involved in the polyol pathway and influences sorbitol levels. An excess of sorbitol can contribute to cellular damage especially in the context of diabetes. SDH’s role is linked to the exclusive conversion of sorbitol to fructose contrasting with aldose reductase which initiates sorbitol production from glucose. Both enzymes are key in maintaining the balance between extracellular and intracellular glucose.\u003cbr\u003e\nAlterations in SDH activity relate to diabetic complications like diabetic retinopathy and neuropathy. The accumulation of sorbitol due to insufficient SDH activity contributes to the development of these conditions. SDH's connection with aldose reductase is particularly notable here as both proteins impact the sorbitol levels that affect various tissues under hyperglycemic conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843588542633,"sku":"ab233613","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab233613","provider":"Iright","version":"1.0","type":"link"}