{"product_id":"abcam-ab234054","title":"Abcam, ab234054, Phagocytosis Assay Kit (Red Zymosan)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nPhagocytosis Assay Kit ab234054 uses Zymosan particles, that are fluorescently labeled with a red dye, to detect and quantify in vitro phagocytosis by fluorescent microscope, fluorometric plate reader or flow cytometry. - Ex\/Em 540\/570 nm - Validated as macrophage phagocytosis assay. - includes labeled Zymosan particles and assay buffer. Also includes quenching solution for readout on microscope \/ plate reader.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay Platform:Microplate reader, Fluor. microscope, Flow cyt.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPhagocytosis Assay Kit ab234054 uses Zymosan particles which have been pre-labeled with a red dye.\u003cbr\u003e\nThe assay can be used for the rapid and accurate detection and quantification of\u003cbr\u003e\nin vitro\u003cbr\u003e\nphagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. It provides a robust screening system for activators and\/or inhibitors of phagocytosis and Toll-like receptors ligands (TLR).\u003cbr\u003e\nThe Zymosan particles used in the phagocytosis assay protocol are labeled for fluorescence at Ex\/Em 540\/570 nm.\u003cbr\u003e\nPhagocytosis assay protocol summary\u003cbr\u003e\n- add Red Zymosan reagent to cells, and incubate for 2-3 hours\u003cbr\u003e\n- harvest cells by centrifugation, remove supernatant, and resuspend cells in Assay Buffer; repeat wash step 3 times\u003cbr\u003e\n- for fluorescent microscope or fluorometric plate reader analysis, use Quenching Solution\u003cbr\u003e\n- analyze cells with flow cytometer, fluorescent microscope, or fluorometric plate reader\u003cbr\u003e\nRelated phagocytosis assays\u003cbr\u003e\nPhagocytosis Assay Kit (Green E.coli)\u003cbr\u003e\nab235900\u003cbr\u003e\nPhagocytosis Assay Kit (Red E. coli)\u003cbr\u003e\nab235901\u003cbr\u003e\nPhagocytosis Assay Kit (Green Zymosan)\u003cbr\u003e\nab234053\u003cbr\u003e\nPhagocytosis Assay Kit (Zymosan, Colorimetric)\u003cbr\u003e\nab211156\u003cbr\u003e\nOther notes\u003cbr\u003e\nThis product was previously called K398 Biovision EZCell™ Phagocytosis Assay Kit (Red Zymosan). Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nIn mammals, phagocytosis by phagocytes (e.g., macrophages, dendritic cells, and neutrophils) is essential for a variety of biological events. Phagocytosis comprises a series of events, starting with the binding and recognition of particles by cell surface receptors, followed by the formation of actin-rich membrane extensions around the particle.\u003cbr\u003e\nZymosan (Saccharomyces cerevisiae) is prepared from yeast cell wall and consists of protein-carbohydrate complexes. Zymosan is a commonly used pathogen in phagocytosis assays.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843629928617,"sku":"ab234054","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab234054","provider":"Iright","version":"1.0","type":"link"}