{"product_id":"abcam-ab234622","title":"Abcam, ab234622, Lysosomal Intracellular Activity Assay Kit","description":"\u003cp\u003eSize: 50Test\u003cbr\u003e\nLysosomal Intracellular Activity Assay Kit (ab234622) provides a proprietary Lysosome-Specific Self-Quenched Substrate which has low background fluorescence, high signal to background ratio and is pH insensitive.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Cell-based,\u003cbr\u003e\nAssay Platform:Flow cytometer, Fluorescence microscope\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nLysosomal Intracellular Activity Assay Kit (ab234622) provides a proprietary Lysosome-Specific Self-Quenched Substrate which has low background fluorescence, high signal to background ratio and is pH insensitive. The substrate, acting as endocytic cargo, can be taken up by cells and degraded within an endo-lysosomal vesicle. The fluorescent signal is recovered from the Self-Quenched Substrate. The fluorescence signal, generated by degradation, is proportional to the intracellular lysosomal activity and can be measured using a fluorescence microscopy and\/or flow cytometry. The kit includes Cytochalasin D, a cell-permeable inhibitor of the lysosomal membrane V-ATPase proton pump, that serves as an anti-lysosomal experimental control. This easy-to-use, non-radioactive kit allows imaging and accurate measurement of de-quenching substrate in cultured cells.\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K448 Lysosomal Intracellular Activity Assay Kit. K448-50 is the same size as the 50 test size of ab234622.\u003cbr\u003e\nLysosomes are membrane-bound organelles important for various cellular processes. They contain hydrolytic enzymes that are utilized in the metabolism of some biomolecules. The extracellular cargo (e.g. nutrients toxins) binds to the cell membrane and is subsequently transported into membrane-bound endosomes for further degradation by lysosomes while intracellular components are transported to lysosomes through autophagy. Lysosomal dysfunction is associated with many human conditions such as aging and neurodegenerative disease.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843611578537,"sku":"ab234622","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab234622","provider":"Iright","version":"1.0","type":"link"}