{"product_id":"abcam-ab235851","title":"Abcam, ab235851, Recombinant Spike glycoprotein (His tag)","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRecombinant Spike glycoprotein (His tag) is a Bovine coronavirus Mebus Fragment protein, in the 326 to 540 aa range, expressed in Escherichia coli, with \u0026gt;85%, suitable for SDS-PAGE, Mass Spec.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;85% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:His tag N-Terminus,\u003cbr\u003e\nApplications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nAccession:P15777,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Bovine coronavirus Mebus,\u003cbr\u003e\nStorage buffer:pH: 7.2 - 7.4Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Spike glycoprotein also known as S protein or surface glycoprotein plays an important role in the mechanism of viral entry into host cells. This protein is important for mediating the fusion of the viral envelope with the host cell membrane facilitating viral entry. It is a trimeric glycoprotein typically expressed on the surface of coronaviruses including SARS-CoV-2 and has a mass of approximately 180 kDa. Each monomer within the trimer structure contains S1 and S2 subunits that are important for host cell attachment and membrane fusion respectively.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis glycoprotein facilitates the attachment of the virus to host cells by binding to specific receptors. The Spike glycoprotein engages with the angiotensin-converting enzyme 2 (ACE2) receptor a step essential for viral entry and infection. It is part of a complex process that involves changes in the conformation of the protein to expose fusion peptides that drive membrane fusion. The structure allows the virus to effectively latch onto the surface of host cells initiating invasion and replication cycles.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe Spike glycoprotein functions in the viral entry pathway a critical mechanism for viral infection. This pathway involves other proteins such as the TMPRSS2 enzyme which aids in the priming of the Spike protein for effective entry. The process of cell entry also associates the glycoprotein with endocytosis where it collaborates with clathrin-mediated pathways for successful internalization. These pathways are integral for the virus’s life cycle and replication within host cells.\u003cbr\u003e\nThe Spike glycoprotein is closely linked with COVID-19 the disease caused by SARS-CoV-2. The interaction between the Spike protein and ACE2 is significant in understanding the viral pathology and the severity of infections. Additionally mutations in the Spike protein can affect transmissibility and immune evasion influencing vaccine efficacy. The glycoprotein’s role in facilitating virus-host interactions implicates its importance in potential treatment strategies targeting viral entry.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843914158249,"sku":"ab235851","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab235851","provider":"Iright","version":"1.0","type":"link"}