{"product_id":"abcam-ab235901","title":"Abcam, ab235901, Phagocytosis Assay Kit (Red E. coli)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nPhagocytosis Assay Kit ab235901 uses heat-killed E. coli particles, that are fluorescently labeled with a red dye, to detect and quantify in vitro phagocytosis by fluorescent microscope, fluorometric plate reader or flow cytometry. - Ex\/Em 540\/570 nm - Validated as macrophage phagocytosis assay. - includes labeled E.coli and assay buffer. Also includes quenching solution for readout on microscope \/ plate reader.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay Platform:Microplate reader, Fluor. microscope, Flow cyt.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPhagocytosis Assay Kit (Red\u003cbr\u003e\nE. coli\u003cbr\u003e\n) (ab235901) uses heat-killed, fluorescently pre-labeled\u003cbr\u003e\nE. coli\u003cbr\u003e\nparticles as a tool for rapid and accurate detection and quantification of\u003cbr\u003e\nin vitro\u003cbr\u003e\nphagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. The kit provides a robust screening system for activators and\/or inhibitors of phagocytosis and Toll-like receptor (TLR) ligands.\u003cbr\u003e\nPhagocytosis assay protocol summary\u003cbr\u003e\n- add Red E. coli reagent to cells, and incubate for 2-3 hours\u003cbr\u003e\n- harvest cells by centrifugation, remove supernatant, and resuspend cells in Assay Buffer; repeat wash step 3 times\u003cbr\u003e\n- for fluorescent microscope or fluorometric plate reader analysis, use Quenching Solution\u003cbr\u003e\n- analyze cells with flow cytometer, fluorescent microscope, or fluorometric plate reader\u003cbr\u003e\nRelated phagocytosis assays\u003cbr\u003e\nPhagocytosis Assay Kit (Green E.coli)\u003cbr\u003e\nab235900\u003cbr\u003e\nPhagocytosis Assay Kit (Green Zymosan)\u003cbr\u003e\nab234053\u003cbr\u003e\nPhagocytosis Assay Kit (Red Zymosan)\u003cbr\u003e\nab234054\u003cbr\u003e\nPhagocytosis Assay Kit (Zymosan, Colorimetric)\u003cbr\u003e\nab211156\u003cbr\u003e\nOther notes\u003cbr\u003e\nThis product was previously called K964 Biovision EZCell™ Phagocytosis Assay Kit (Red E. coli). Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nPhagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides.\u003cbr\u003e\nE. coli\u003cbr\u003e\nand other bacterial strains are often used as a pathogen in phagocytosis assays.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855621345449,"sku":"ab235901","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab235901","provider":"Iright","version":"1.0","type":"link"}