{"product_id":"abcam-ab235976","title":"Abcam, ab235976, 2-NBDG Glucose Uptake Assay Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\n2-NBDG Glucose Uptake Assay Kit ab235976 provides a convenient tool for studying cellular glucose uptake in cell cultures. - includes glucose uptake inhibitor, and also propidium iodide to exclude dead cells - use with standard fluorometric plate reader, fluorescent microscope or flow cytometer; Ex\/Em 485\/535 nm\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Adherent cells, Suspension cells,\u003cbr\u003e\nAssay type:Cell-based (quantitative),\u003cbr\u003e\nAssay Platform:Microplate reader, Fluor. microscope, Flow cyt.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\n2-NBDG Glucose Uptake Assay Kit ab235976 provides a convenient tool for studying cellular glucose uptake. The kit uses 2-NBDG, a fluorescently-labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells.\u003cbr\u003e\nThe 2-NBDG assay kit is suitable for analysis with flow cytometer, fluorescent microscope or microplate reader.\u003cbr\u003e\n2-NBDG protocol summary:\u003cbr\u003e\n- treat cells with experimental treatment in glucose-free medium\u003cbr\u003e\n- add 2-NBDG and incubate for 10 min\u003cbr\u003e\n- remove cell culture supernatant and wash cells with cell-based assay buffer\u003cbr\u003e\n- add propidium iodide at this point to exclude dead cells if analyzing with flow cytometry\u003cbr\u003e\n- add cell-based assay buffer and analyze immediately\u003cbr\u003e\n2-NBDG can be detected with filters designed to detect FITC (Ex\/Em 485\/535 nm).\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843618754729,"sku":"ab235976","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab235976","provider":"Iright","version":"1.0","type":"link"}