{"product_id":"abcam-ab236639","title":"Abcam, ab236639, Anti-RUNX2 antibody [EPR22858-106] - ChIP Grade","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-RUNX2 antibody [EPR22858-106] - ChIP Grade (ab236639) is a rabbit monoclonal antibody detecting RUNX2 in  Western Blot, IP, IHC-P, ChIP . Suitable for  Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 60 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR22858-106,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:ChIP, IHC-P, WB, IP, ChIC\/CUT\u0026amp;RUN-seqSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:ab236639 is not recommended for mouse IHC.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-RUNX2 antibody [EPR22858-106] - ChIP Grade (ab236639) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), ChIP in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of RUNX2?\u003cbr\u003e\nAnti-RUNX2 [EPR22858-106] - ChIP Grade (ab236639) specifically detects a band for RUNX2 (UniProt: Q13950) at a molecular weight of 57kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-RUNX2 [EPR22858-106] - ChIP Grade (ab236639) was first used in a scientific publication in 2019 and has been cited over 60 times in peer-reviewed journals.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR22858-106] also available for your convenience: ab236639, Carrier free -\u003cbr\u003e\nab264077\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab275219\u003cbr\u003e\n, PE -\u003cbr\u003e\nab318316\u003cbr\u003e\n, APC -\u003cbr\u003e\nab318419\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab318522\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab318625\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab318728\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab318828\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab321481\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRUNX2 also known as core-binding factor subunit alpha-1 (CBFA1) is a transcription factor with a molecular weight of approximately 56 kDa. It plays a pivotal role in regulating osteoblast differentiation and skeletal morphogenesis. This protein resides mainly in the nucleus and shows a high expression in bone tissue. RUNX2 binds to DNA sequences to control transcription of genes essential for bone development and maturation. The protein also possesses a Runt-homology domain enabling it to attach to specific DNA sequences.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein orchestrates bone formation by influencing the expression of genes involved in osteoblast differentiation. RUNX2 operates as part of the larger transcriptional complex that includes other proteins that modulate its activity. It maintains bone homeostasis by regulating the expression of osteogenic markers including bone sialoprotein and osteocalcin. The ability of RUNX2 to drive osteoblast lineage commitment highlights its importance in skeletal health and development.\u003cbr\u003e\nPathways\u003cbr\u003e\nRUNX2 shows significant involvement in both the Wnt signaling and TGF-beta signaling pathways. Within the Wnt signaling pathway RUNX2 interacts with beta-catenin to facilitate osteoblastogenesis and support bone matrix deposition. In the TGF-beta signaling pathway RUNX2 modulates Smad-dependent transcriptional activity important for extracellular matrix production and bone remodeling. These interactions illustrate RUNX2's central role in mediating bone development and maintenance.\u003cbr\u003e\nRUNX2 mutations and dysregulation link to cleidocranial dysplasia a condition characterized by delayed closure of sutures in the skull and other skeletal anomalies. Additionally RUNX2 is related to osteosarcoma a type of bone cancer where its abnormal expression often contributes to tumor progression. In these contexts RUNX2 interacts with proteins like p53 an important regulator of cell cycle highlighting its influence on tumor suppressor networks and osteogenic pathways.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855707295913,"sku":"ab236639","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab236639","provider":"Iright","version":"1.0","type":"link"}