{"product_id":"abcam-ab240087","title":"Abcam, ab240087, Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal HLA A antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB, ICC\/IF and reacts with Human samples. Cited in 3 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR1394Y,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IHC-P, Flow Cyt, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:In our hands this antibody did not work in WB with mouse samples, but customer data suggest that reactivity in ICC\/IF and IHC. The target shows high sequence homology with HLA-C and HLA-E, and it is possible that this antibody will detect these proteins too.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab240087 is the carrier-free version of\u003cbr\u003e\nab134189\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nRat: We have preliminary internal testing data to indicate this antibody may not react with this species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMHC class I molecules including HLA-A and HLA-B are proteins integral to the immune system. They often refer to as HLA which stands for Human Leukocyte Antigens. Typically MHC class I proteins with a molecular mass of around 45 kDa are expressed on the surface of nearly all nucleated cells. Their main mechanical function is to present endogenous peptides to CD8+ cytotoxic T lymphocytes enabling the immune system to monitor and respond to intracellular changes such as infections or malignancies.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThese MHC molecules play a critical role in adaptive immunity. They form a complex with beta-2 microglobulin (β2m) to achieve stability and function properly on cell surfaces. HLA-A and HLA-B bind to short peptides derived from intracellular proteins which are then transported through the endoplasmic reticulum. The peptide-MHC complex is then expressed on the cell surface where it facilitates recognition by T cell receptors guiding immune responses against infected or transformed cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nMHC class I molecules are deeply involved in the antigen processing and presentation pathway as well as the immune effector process. In the antigen processing pathway proteins within the cell undergo degradation by the proteasome creating peptide fragments. These fragments are transported into the endoplasmic reticulum by the TAP (transporter associated with antigen processing) proteins where they bind with MHC class I forming a stable complex. Proteins like TAP and proteasome subunits work closely with MHC class I to ensure proper immune surveillance.\u003cbr\u003e\nDysregulation or alterations in MHC class I molecules particularly HLA-A and HLA-B have been linked to autoimmune diseases and cancers. For instance specific HLA alleles associate with autoimmune conditions like Type 1 Diabetes where the immune system erroneously targets pancreatic cells. In cancers a downregulation of MHC class I molecules often occurs as a mechanism to evade immune detection. Altered expression of proteins like TAP can also disrupt antigen presentation contributing to the disease onset and progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46856217985193,"sku":"ab240087","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab240087","provider":"Iright","version":"1.0","type":"link"}