{"product_id":"abcam-ab241082","title":"Abcam, ab241082, Fluo-4 AM, Fluorescent labeling reagent","description":"\u003cp\u003eSize: 10 x 50µg \/ 1mg\u003cbr\u003e\nFluo-4 AM (ab241082) is a cell permeable, green fluorescent calcium probe used to study intracellular free calcium concentrations in fluorescence microscopy and flow cytometry. - Ex\/Em: 495\/528 nm - Available in different sizes to fit your experimental needs\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:Chemical Structure, Fluorescent Cell ImagingSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nTarget:Fluo-4 amSee target data,\u003cbr\u003e\nPurity:\u0026gt;98%,\u003cbr\u003e\nForm:SolidSee storage information,\u003cbr\u003e\nStorage buffer:Constituents: 100% Fluo-4 AM\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCheck out our range of Fluo AM dyes\u003cbr\u003e\nRelated Products:\u003cbr\u003e\nCalcium indicators and ionophores\u003cbr\u003e\nProtocol:\u003cbr\u003e\n1. On the day of the experiment, either dissolve Fluo-4 AM in DMSO or thaw an aliquot of the indicator stock solution to room temperature.\u003cbr\u003e\n2. Prepare a 2 to 20 µM Fluo-4 AM working solution in a buffer of your choice (e.g., Hanks and Hepes buffer) with 0.04% Pluronic\u003cbr\u003e\nF-127. For most cell lines, Fluo-4 AM at a final concentration of 4-5 μM is recommended. The exact concentration of indicators required for cell loading must be determined empirically.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Store in the dark, Store under desiccating conditions\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847574311081,"sku":"ab241082","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab241082","provider":"Iright","version":"1.0","type":"link"}