{"product_id":"abcam-ab241922","title":"Abcam, ab241922, Human Haptoglobin Antibody Pair - BSA and Azide free","description":"\u003cp\u003eSize: 10 x 96Tests\u003cbr\u003e\nHuman Haptoglobin Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Haptoglobin.\u003cbr\u003e\nKey facts\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:ELISA set,\u003cbr\u003e\nRange:312.5 - 20000 pg\/mL,\u003cbr\u003e\nAssay Platform:Reagents\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThe Antibody Pair can be used to quantify Human Haptoglobin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg\/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.\u003cbr\u003e\nFor additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (\u003cbr\u003e\nab219048\u003cbr\u003e\n), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nUse our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with \u0026lt;1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nThe pair can be used in variety of assays and platforms including but not limited to:\u003cbr\u003e\nSandwich ELISA\u003cbr\u003e\nFRET\/TR-FRET\/HTRF\u003cbr\u003e\nMeso Scale Discovery\u003cbr\u003e\nBead-based assays\u003cbr\u003e\nAlphaLISA\u003cbr\u003e\n\/AlphaScreen\u003cbr\u003e\nDELFIA\u003cbr\u003e\nimmunoassays\u003cbr\u003e\nand Single Molecule Counting (SMC™) immunoassays\u003cbr\u003e\nMultiplex\u003cbr\u003e\nOur antibody pairs are supplied in a carrier-free format that is conjugation-ready:\u003cbr\u003e\nBuffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.\u003cbr\u003e\nConcentration of ~1 mg\/ml as measured by the protein A280 method.\u003cbr\u003e\nWe can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.\u003cbr\u003e\nPairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.\u003cbr\u003e\nPlease note:\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.\u003cbr\u003e\nThe range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.\u003cbr\u003e\nWe guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.\u003cbr\u003e\nAntibody properties:\u003cbr\u003e\nCapture antibody:\u003cbr\u003e\nrecombinant rabbit monoclonal (unconjugated) – 100 µg\u003cbr\u003e\nDetector antibody:\u003cbr\u003e\nrecombinant rabbit monoclonal (unconjugated) - 100 µg\u003cbr\u003e\nConcentration\u003cbr\u003e\n: ~1 mg\/ml\u003cbr\u003e\nStorage buffer:\u003cbr\u003e\n100% PBS\u003cbr\u003e\nLiquid\u003cbr\u003e\nIsotype:\u003cbr\u003e\nRecombinant monoclonal antibodies offer several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free production\u003cbr\u003e\nMeso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.\u003cbr\u003e\nAlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.\u003cbr\u003e\nSimoa is a registered trademark of Quanterix, Inc.\u003cbr\u003e\nSMC is a registered trademark of Merck KGaA, Darmstadt, Germany.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHaptoglobin (Hp) also known as Hp protein is a glycoprotein with a molecular weight ranging from 85 to 100 kDa varying due to its ability to form different dimeric and multimeric structures. It is primarily synthesized in the liver and circulating in the plasma. Mechanically haptoglobin binds free hemoglobin released from erythrocytes preventing oxidative damage and hemoglobin-driven deleterious effects. Haptoglobin's binding to hemoglobin forms a haptoglobin-hemoglobin complex that gets cleared by the CD163 receptor on macrophages mainly in the liver and spleen.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHaptoglobin functions as an antioxidant and an acute phase protein playing roles in inflammation and immune response. It is an essential component of the antioxidant defense system reducing oxidative stress by binding free hemoglobin. Additionally haptoglobin can participate in complex formation with hemoglobin to facilitate the clearance of excess free hemoglobin from circulation. Its expression increases during inflammation under the regulation of cytokines like IL-6.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe haptoglobin protein is part of the hemoglobin clearance pathway integral for iron metabolism and homeostasis. This pathway ensures the safe removal of hemoglobin from the blood helping to prevent kidney damage. Haptoglobin is related to other proteins such as CD163 and the liver-derived hepatocellular proteins involved in the clearance process. Also haptoglobin is linked to antioxidant pathways collaborating with oxidative stress regulators to manage reactive oxygen species levels.\u003cbr\u003e\nChanges in haptoglobin levels are associated with hemolytic anemia and inflammatory conditions. Low haptoglobin levels often suggest hemolytic anemia due to its rapid binding with free hemoglobin in the bloodstream. During inflammation and infections 'anti-Hp' tests (haptoglobin tests) may show increased haptoglobin levels as a response to cytokine signaling. Moreover haptoglobin polymorphisms have a connection with ischemic stroke susceptibility where its isoforms show differing efficiencies in binding free hemoglobin. These findings highlight haptoglobin's relevance as a biomarker for these conditions and illustrate its interaction with hematologic and immune proteins in disease contexts.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843607023785,"sku":"ab241922","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab241922","provider":"Iright","version":"1.0","type":"link"}