{"product_id":"abcam-ab245660","title":"Abcam, ab245660, Anti-DIAPH3 antibody","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRabbit Polyclonal DIAPH3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human DIAPH3 aa 1100 to C-terminus.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human DIAPH3 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9NSV4\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab245660 was affinity purified using an epitope specific to DIAPH3 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate\/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDIAPH3 also known as diaphanous-related formin 3 is a protein with an approximate mass of 130 kDa. It plays an important role in the regulation of the actin cytoskeleton essential for the dynamic reorganization of actin filaments. DIAPH3 primarily functions by mediating actin nucleation and elongation facilitating cellular processes such as shape changes adhesion and division. The protein is expressed in various tissues with notable expression in the brain testis and pancreas.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nDIAPH3 is a part of the formin family and acts by promoting the polymerization of actin filaments. It forms a complex with profilin and other actin-binding proteins maintaining the balance of cellular architecture. DIAPH3 contributes to the maintenance of normal cellular structure and dynamics participating in cell migration and morphogenesis. Its interaction with GTPases like RhoA regulates the initiation and stabilization of actin filaments directly impacting cell mechanics.\u003cbr\u003e\nPathways\u003cbr\u003e\nDIAPH3 engages in the Rho GTPase signaling pathway impacting actin dynamics and playing roles in cytoskeletal remodelling. It also participates in the mTOR signaling pathway influencing cell growth and metabolism. Within these pathways DIAPH3 works closely with proteins such as mTOR contributing to cellular responses like growth proliferation and survival. These pathways highlight DIAPH3’s involvement in coordinating cellular structural integrity and function.\u003cbr\u003e\nResearch has implicated DIAPH3 in sensory hearing loss and cancer progression. In hearing loss mutations in DIAPH3 disrupt the organization of actin filaments critical for auditory function. In the context of cancer abnormal DIAPH3 expression relates to increased cellular motility and invasion facilitating metastasis. In these conditions DIAPH3 interacts with proteins such as E-cadherin affecting cellular adhesion and communication and emphasizing its significance in disease mechanisms.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855817855145,"sku":"ab245660","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab245660","provider":"Iright","version":"1.0","type":"link"}