{"product_id":"abcam-ab246547","title":"Abcam, ab246547, Mouse MCP-5 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse MCP-5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MCP-5 with a sensitivity of 6 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Citrate plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 6 pg\/mL,\u003cbr\u003e\nRange:27.56 - 1700 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse MCP-5 ELISA Kit (ab246547) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP-5 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MCP-5 with 6 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n- Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n- High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n- Fully validated in biological samples\u003cbr\u003e\n- 96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMCP-5 also known as CCL12 is a chemokine protein that plays a role in immune response by recruiting and activating leukocytes. It has a molecular mass of approximately 12 kDa. This protein is expressed mainly in tissues related to the immune system including the spleen and liver. MCP-5 exhibits a sequence homology with other members of the C-C chemokine family and shares functional similarities with MCP-1.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMCP-5 takes part in promoting the migration of monocytes and lymphocytes to sites of inflammation. This chemokine is not a part of a complex but interacts with specific receptors on target cells such as CCR2. Through these interactions MCP-5 helps mediate a range of immune responses enhancing both innate and adaptive immune activities. It also contributes to the clearance of pathogens by guiding leukocytes to infection sites.\u003cbr\u003e\nPathways\u003cbr\u003e\nMCP-5 plays an important role in the chemokine signaling pathway and the inflammatory response pathway. Through chemokine signaling MCP-5 interacts with CCR2 facilitating the movement of immune cells to areas of tissue damage or infection. This regulation of cell traffic is essential for maintaining homeostasis and responding to pathological changes in tissues. Another protein connected to MCP-5 in these pathways is MCP-3 which shares overlapping functions in immune cell recruitment.\u003cbr\u003e\nMCP-5 has been implicated in conditions such as asthma and rheumatoid arthritis. In these disorders MCP-5 contributes to the recruitment of inflammatory cells worsening symptoms. It also shows a connection with other proteins like MCP-1 which similarly aids in the movement of monocytes to inflammatory sites. The presence and activity of MCP-5 and its related proteins can therefore represent potential targets for therapeutic interventions in controlling excessive inflammatory responses.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843581857961,"sku":"ab246547","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab246547","provider":"Iright","version":"1.0","type":"link"}