{"product_id":"abcam-ab247860","title":"Abcam, ab247860, Anti-NEK7 antibody [EPR4901] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nAnti-NEK7 antibody [EPR4901] - BSA and Azide free (ab247860) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting NEK7 in  Western Blot, Flow Cytometry (Intra), Flow Cytometry . Suitable for  Human . - KO validated for confirmed specificity - BSA, sodium azide, and glycerol-free for easy conjugation - Biophysical QC for unrivalled batch-batch consistency\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR4901,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-NEK7 antibody [EPR4901] - BSA and Azide free (ab247860) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human samples.\u003cbr\u003e\nWhat is the molecular weight of NEK7?\u003cbr\u003e\nAnti-NEK7 [EPR4901] - BSA and Azide free (ab247860) specifically detects a band for NEK7 (UniProt: Q8TDX7) at a molecular weight of 35kDa.\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Anti-NEK7 antibody [EPR4901] - BSA and Azide free (ab247860) has been confirmed by Flow Cytometry (Intracellular) testing in NEK7 Knockout HAP1 cells.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR4901] also available for your convenience:\u003cbr\u003e\nab109433\u003cbr\u003e\n, Carrier free - ab247860\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe NIMA-related kinase 7 (NEK7) is a member of the NEK family of serine\/threonine kinases and plays an important role in cell division. With a molecular mass of approximately 34 kDa the NEK7 protein is involved in mitosis by regulating microtubule organization. It is primarily expressed in various tissues including human and marmoset thyroid tissues. It remains mainly inactive until mitotic entry where its activity becomes essential for proper spindle formation and completion of mitosis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCell cycle progression heavily depends on NEK7's function. NEK7 interacts with NEDD1 in the microtubule-organizing center contributing to centrosome maturation and separation. This interaction makes NEK7 vital in the recruitment of γ-tubulin ring complex which is necessary for nucleating microtubules. NEK7 operates independently from the other NEK family members but shares involvement in centrosome-related activities.\u003cbr\u003e\nPathways\u003cbr\u003e\nNEK7 is influential in the regulation of the mitotic spindle assembly pathway and the interleukin-1 (IL-1) signaling pathway. It is closely related to proteins like NEK6 and NEK9 which also play roles in mitotic progression and cytokinesis. NEK7's involvement in these pathways indicates its integrative function in ensuring normal cell division and response to inflammatory signals.\u003cbr\u003e\nNEK7 has implications in cancer and inflammatory ailments. NEK7 overexpression or mutation is often linked to tumorigenesis particularly in lung and brain cancers. Additionally NEK7 contributes to the activation of the NLRP3 inflammasome connecting it to inflammatory conditions like rheumatoid arthritis. The interplay with NLRP3 highlights the significance of NEK7 in inflammation-related diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851906797737,"sku":"ab247860","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab247860","provider":"Iright","version":"1.0","type":"link"}