{"product_id":"abcam-ab248327","title":"Abcam, ab248327, Anti-MED4 antibody [EPR7816] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal MED4 antibody. Carrier free. Suitable for IHC-P, ICC\/IF, WB and reacts with Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR7816,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-P, WB, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab248327 is the carrier-free version of\u003cbr\u003e\nab129170\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMED4 also known as mediator complex subunit 4 plays an important role in transcription regulation. MED4 forms part of the larger mediator complex which interacts with RNA polymerase II during the transcription initiation process. This protein has a mass of approximately 35 kilodaltons. Researchers have detected MED4 expression in various tissues with notable levels in the liver and lung implying its broad involvement in different cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMED4 operates as an integral component of the mediator complex a multi-protein machinery that transmits signals from transcription factors to RNA polymerase II facilitating the transcription of specific genes. This mediator complex acts as a bridge affecting transcription initiation and further steps. MED4's presence within this complex highlights its importance in regulating gene expression and maintaining normal cell function.\u003cbr\u003e\nPathways\u003cbr\u003e\nMED4 directly contributes to the RNA polymerase II transcription initiation complex pathway. This pathway is important for transcribing genes into mRNA. MED4 also engages in chromatin-related processes through its mediator role influencing the transcription machinery's interaction with chromatin structures. Connections between MED4 and other proteins like MED1 and MED12 highlight its collaborative function in transcription regulation.\u003cbr\u003e\nAbnormal MED4 function associates with certain types of cancer including breast cancer. Research suggests that dysfunctional MED4 can disrupt typical transcriptional regulation leading to uncontrolled cell proliferation. Additionally alterations in MED4 have links to neurological disorders as they may affect gene expression necessary for normal brain function. MED4's relationship with proteins like p53 in cancer further highlights its role in disease pathways.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851740762281,"sku":"ab248327","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab248327","provider":"Iright","version":"1.0","type":"link"}