{"product_id":"abcam-ab248388","title":"Abcam, ab248388, Anti-CPSF73 antibody [EPR7468] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal CPSF73 antibody. Carrier free. Suitable for ICC\/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR7468,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:ICC\/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab248388 is the carrier-free version of\u003cbr\u003e\nab131245\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCPSF73 also known as Cleavage and Polyadenylation Specificity Factor 73 is an important component of the mRNA processing machinery. Mechanically CPSF73 functions as an endonuclease that cleaves pre-mRNA at the 3' end a necessary step for the addition of a poly(A) tail. CPSF73 has a molecular mass of approximately 73 kDa. This protein is expressed ubiquitously across various cell types reflecting its essential role in gene expression regulation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe mRNA processing involves CPSF73 as part of the larger cleavage and polyadenylation complex. This complex is responsible for recognizing specific cleavage sites within pre-mRNA and is critical for mRNA maturation. CPSF73’s activity ensures the proper maturation of messenger RNA facilitating its export from the nucleus translation into proteins and subsequent degradation. The endonuclease works closely with other components of the complex including CPSF160 CPSF100 and CFIm suggesting that its role extends beyond just cleavage to broader mRNA stability and regulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nCPSF73 actively participates in the mRNA processing pathway playing an integral role in post-transcriptional regulation. It also interfaces with the spliceosome machinery illustrating the crosstalk between pre-mRNA splicing and polyadenylation sites. CPSF73 is closely related to other RNA processing proteins such as CstF-64 and Symplekin which together maintain the precise regulation of gene expression. This interrelation is particularly important in pathways where timing of mRNA processing impacts cellular function and response.\u003cbr\u003e\nCPSF73's malfunction could lead to serious implications including cancer and genetic disorders. Aberrant expression or mutations in CPSF73 have been linked to various cancers as they directly affect mRNA maturation and stability which could result in unchecked cell proliferation. Furthermore its connection with proteins such as CPSF160 has been observed in cancers where dysregulation of mRNA processing pathways contribute to tumorigenesis. Disorders like these highlight the necessity of CPSF73 for maintaining cellular health and proper gene expression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46856368619689,"sku":"ab248388","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab248388","provider":"Iright","version":"1.0","type":"link"}