{"product_id":"abcam-ab248432","title":"Abcam, ab248432, Anti-RUNX1 \/ AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal RUNX1 \/ AML1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR3098,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nApplications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:The immunogen of this antibody is 100% homologous to mouse RUNX2 sequence.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab248432 is the carrier-free version of\u003cbr\u003e\nab133261\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nRat: We have preliminary internal testing data to indicate this antibody may not react with this species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRUNX1 also known as AML1 is a transcription factor that plays an important role in gene regulation. RUNX3 and RUNX2 are related proteins that belong to the same RUNX family which are defined by the runt homology domain. RUNX1 typically has a mass of approximately 50 kilodaltons (kDa). These proteins express in various tissues including hematopoietic cells for RUNX1 skeletal tissues for RUNX2 and immune cells for RUNX3. They bind to DNA influencing the transcription of target genes which are critical for development processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRUNX proteins mediate cellular differentiation and proliferation. They often participate in complex with CBFβ which stabilizes their interaction with DNA and enhances transcriptional activity. RUNX1 in particular regulates hematopoiesis the process of blood cell formation. RUNX2 is fundamentally involved in osteogenesis facilitating bone growth and remodeling. RUNX3 contributes to immune responses by affecting T-cell differentiation and function. Their dynamic interactions dictate the expression levels of genes involved in these vital processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nRUNX proteins integrate into significant signaling cascades. In hematopoietic regulation RUNX1 interacts importantly within the Notch and TGF-β pathways. It cooperates with other proteins such as GATA1 and TAL1 influencing lineage commitment and differentiation of blood cells. RUNX2 critical in the ossification pathway often partners with proteins including SMADs in response to bone morphogenetic protein (BMP) signaling guiding osteoblast differentiation. These pathways demonstrate the versatility and specificity of RUNX proteins in biological processes.\u003cbr\u003e\nAberrations in RUNX1 are strongly linked to acute myeloid leukemia (AML). Mutations or translocations affecting RUNX1 disrupt normal hematopoiesis leading to leukemogenic processes. RUNX3 involvement can be observed in cancers like gastric cancer where its silencing or inactivation associates with tumorigenesis. RUNX2 overexpression sometimes links with bone disorders such as cleidocranial dysplasia. Here RUNX2 may interact with other skeletal regulators like PTHLH influencing proper bone development and growth. Understanding these interactions provides insights into potential therapeutic targets for managing these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851765567657,"sku":"ab248432","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab248432","provider":"Iright","version":"1.0","type":"link"}