{"product_id":"abcam-ab250071","title":"Abcam, ab250071, Anti-ALG2 antibody [EPR12006(2)(B)] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal ALG2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR12006(2)(B),\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab250071 is the carrier-free version of\u003cbr\u003e\nab178697\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nMouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe ALG2 protein also known as asparagine-linked glycosylation 2 plays a role in the early steps of N-glycan precursor biosynthesis. ALG2 has a molecular mass of approximately 51 kDa and shows expression in multiple tissues including the liver and brain. The protein acts mechanically to facilitate the addition of the first mannose residue to the lipid-linked oligosaccharide (LLO) precursor in the endoplasmic reticulum a fundamental step in protein glycosylation processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCertain processes depend on the actions of ALG2. This protein becomes part of the larger oligosaccharyltransferase complex where it ensures proper glycosylation of nascent proteins—a modification important for protein folding and stability. ALG2 modulates the efficiency of protein maturation by its role in glycan formation affecting how proteins interact within cellular processes. Defects in ALG2 lead to improper protein folding and cellular distribution.\u003cbr\u003e\nPathways\u003cbr\u003e\nALG2 functions as an important component in the protein glycosylation pathway and more specifically in the N-glycan assembly pathway. This protein interacts closely with other glycosylation enzymes like ALG12 which further add branches to the glycans once ALG2 completes its function. The interactions within this pathway ensure proper biological functionalization of glycoproteins facilitating diverse roles from cell signaling to immune recognition.\u003cbr\u003e\nALG2 mutations have known associations with congenital disorders of glycosylation (CDG) a group of diseases caused by defects in glycosylation. Such mutations can cause a spectrum of developmental and metabolic issues. ALG2 dysfunction linked to proteins like ALG9 in co-regulatory roles reveals the impact of glycosylation on cellular homeostasis. These insights into ALG2 give promise for potential therapeutic targets in managing CDG symptoms and advancing understanding of glycosylation-related pathology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848569933993,"sku":"ab250071","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab250071","provider":"Iright","version":"1.0","type":"link"}