{"product_id":"abcam-ab250150","title":"Abcam, ab250150, Anti-APPL antibody [EPR13569] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal APPL antibody. Carrier free. Suitable for ICC\/IF, WB, IHC-P and reacts with Human samples. Cited in 2 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR13569,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IHC-P, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab250150 is the carrier-free version of\u003cbr\u003e\nab180140\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAPPL also known as Adaptor Protein Phosphotyrosine Interaction PH Domain and Leucine Zipper Containing 1 (APPL1) is an adaptor protein that plays an important role in cellular signal transduction. APPL1 has a molecular mass of approximately 80 kDa. It is widely expressed in various tissues including the brain liver and skeletal muscle. APPL1 binds to many other proteins via its pleckstrin homology (PH) domain and a leucine zipper domain playing a role in diverse signaling pathways.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAPPL1 influences cellular processes such as endocytosis and cell proliferation. APPL1 interacts with rab5 forming a component of endosomal signaling complexes. This interaction drives the early endocytic process influencing cell surface receptor trafficking. APPL1 also modulates insulin signaling by binding to proteins like the insulin receptor substrate affecting glucose metabolism and cellular energy balance.\u003cbr\u003e\nPathways\u003cbr\u003e\nAPPL1 acts in the AKT signaling pathway and the adiponectin signaling pathway. In the AKT pathway APPL1 operates with proteins like AKT itself and GSK3β contributing to cell survival and growth. It assists the adiponectin pathway by facilitating interactions between adiponectin receptors and downstream effectors impacting lipid metabolism and insulin sensitivity. APPL1's involvement in these pathways highlights its multifaceted regulatory roles in cellular metabolism and response to stimuli.\u003cbr\u003e\nAPPL1 exhibits associations with type 2 diabetes and certain cancers. APPL1 influences insulin sensitivity a critical factor in type 2 diabetes pathogenesis. It interacts with proteins like adiponectin and the insulin receptor substrate playing a part in glucose homeostasis. In cancer APPL1's role in AKT signaling can influence tumor growth and survival linking it to dysregulation in cancers such as breast cancer. The ability of APPL1 to modulate key signaling pathways highlights its importance in understanding these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46860422414505,"sku":"ab250150","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab250150","provider":"Iright","version":"1.0","type":"link"}