{"product_id":"abcam-ab250224","title":"Abcam, ab250224, Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Spry-2 antibody. Carrier free. Suitable for ICC\/IF, WB, IHC-P and reacts with Human, Rat samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR4318(2)(B),\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human, Rat,\u003cbr\u003e\nApplications:WB, IHC-P, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab250224 is the carrier-free version of\u003cbr\u003e\nab180527\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSpry-2 also known as Sprouty homolog 2 is an intracellular protein involved in the regulation of signaling pathways. It has a molecular weight of approximately 35 kDa. This protein expresses in tissues such as the lung kidney and colon. Mainly Spry-2 acts through interaction with other proteins to inhibit or modulate various cellular processes such as growth and differentiation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSprouty homolog 2 functions by mediating negative feedback in signaling pathways. It plays a role in controlling cellular responses to growth factors. Although not part of a large protein complex it interacts with specific proteins to exert its biological effects. For instance Spry-2 interacts with components of the receptor tyrosine kinase signaling pathway. This interaction helps fine-tune cell signaling responses preventing excessive cell proliferation.\u003cbr\u003e\nPathways\u003cbr\u003e\nSprouty homolog 2 acts within the MAPK\/ERK signaling pathway and the fibroblast growth factor (FGF) pathway. In these pathways Spry-2 functions as an inhibitor thereby maintaining proper cellular regulation. It interacts with proteins like Raf and inhibits their activities ensuring balanced signal transduction. In the MAPK\/ERK pathway this regulation by Spry-2 helps control cellular responses to external stimuli.\u003cbr\u003e\nAbnormalities in Sprouty homolog 2 function relate to cancer and congenital anomalies. Altered expression or mutations in Spry-2 have associations with colorectal cancer partly due to its role in cell signaling regulation. Spry-2 connects to proteins like Ras known for their involvement in oncogenic pathways. Similarly variations in Spry-2 activity can lead to developmental disorders highlighting its importance in normal cellular function.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851741286569,"sku":"ab250224","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab250224","provider":"Iright","version":"1.0","type":"link"}