{"product_id":"abcam-ab250381","title":"Abcam, ab250381, Anti-IREB2\/IRP2 + Aconitase 1\/ACO1 antibody [EPR13854] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal IREB2\/IRP2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR13854,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:The antibody immunogen share 80% homology with the IREB1 protein, therefore it is likely that the antibody cross-react with IREB1. We received some customers feedback where they observed this cross-reactivity, however no experiments were performed in our lab in order to confirm this.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab250381 is the carrier-free version of\u003cbr\u003e\nab181153\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nIREB2 also known as IRP2 and ACO1 also known as Aconitase 1 serve vital functions in iron metabolism. Mechanically IREB2\/IRP2 acts as an iron-responsive element-binding protein and is involved in the post-transcriptional regulation of key genes including those encoding transferrin receptor and ferritin. ACO1 functions as a bifunctional protein that switches between being an enzyme and a regulatory protein based on iron levels known to be around 98 kDa in mass. Both of these proteins express in various tissues highlighting their importance across different biological systems.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe IREB2\/IRP2 and ACO1 maintain cellular iron homeostasis by responding to iron levels and regulating the iron-responsive element system. They do not form part of a larger protein complex but operate independently to modulate iron metabolism. When iron is ample ACO1 behaves as an enzyme catalyzing the isomerization of citrate to isocitrate in the citric acid cycle. Conversely under iron-depleted conditions ACO1 joins IREB2\/IRP2 in an RNA-binding form to control iron uptake and storage.\u003cbr\u003e\nPathways\u003cbr\u003e\nThese proteins play significant roles in the iron regulatory network and the citric acid cycle. IREB2\/IRP2 associates with pathways involving iron uptake and release influencing genes related to ferritin and transferrin receptor. ACO1's dual function ties it to both the citric acid cycle and iron regulatory pathways showing its involvement in cellular energy production and iron balance. The interaction with other important proteins such as transferrin and ferritin is important for their regulatory functions within these pathways.\u003cbr\u003e\nIREB2 and ACO1 connections are apparent in conditions like neurodegenerative diseases and anemia. Abnormal regulation or mutations in these proteins show associations with disorders such as Parkinson’s disease and sideroblastic anemia. Interactions between IREB2\/IRP2 and proteins like PARK7\/DJ-1 and oxidative stress proteins underline their significance in the pathogenesis of neurodegenerative diseases and issues of iron mismanagement.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851916365993,"sku":"ab250381","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab250381","provider":"Iright","version":"1.0","type":"link"}