{"product_id":"abcam-ab250580","title":"Abcam, ab250580, Anti-Chymotrypsin antibody [EPR13860] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Chymotrypsin antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR13860,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab250580 is the carrier-free version of\u003cbr\u003e\nab182137\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nChymotrypsin also known as chymotrypsinogen when in its inactive precursor form and chymotrypsine stands out as a serine protease enzyme with a molecular weight around 25 kDa. Mechanically chymotrypsin catalyzes the hydrolysis of peptide bonds especially those containing aromatic amino acids like phenylalanine tyrosine and tryptophan. The enzyme exhibits a preference for cleaving at the carboxyl side of these amino acids. Chymotrypsin is mainly expressed in the acinar cells of the pancreas where it aids in the digestive process. The enzyme becomes active upon cleavage by trypsin another serine protease forming chymotrypsin and trypsin as active proteolytic enzymes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe enzyme plays an essential role in the digestion of proteins within the small intestine. Chymotrypsin contributes significantly to breaking down dietary proteins into smaller peptides and amino acids which are easier to absorb. During this process chymotrypsin partners with other pancreatic enzymes such as trypsin making up a proteolytic cascade. Chymotrypsin activity ensures efficient protein digestion which is important for nutrient absorption and metabolism. The enzyme's product which results from substrate cleavage is integral to various physiological processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nChymotrypsin is intricately involved in the digestive enzymatic cascade that ensures proper digestion and absorption of nutrients. The proteolytic activity of chymotrypsin and trypsin in the digestive system forms part of the broader system of exocrine pancreatic secretion. This pathway which involves various enzymes like elastase and chemotrypsin facilitates the efficient processing of proteins and is critical for maintaining metabolic homeostasis. Chymotrypsin's interaction with other enzymes highlights its significance in these pathways.\u003cbr\u003e\nAbnormal chymotrypsin activity can link to certain pancreatic disorders such as chronic pancreatitis where enzyme secretion disrupts. This disruption may relate through the dysfunction of other enzymes including trypsin. Moreover hereditary pancreatitis associates with mutations affecting the regulation of the enzyme or its precursor chymotrypsinogen. Understanding these associations helps to elucidate the role of chymotrypsin protein in various pathological states shedding light on the importance of maintaining its normal function to prevent disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850600698025,"sku":"ab250580","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab250580","provider":"Iright","version":"1.0","type":"link"}