{"product_id":"abcam-ab250896","title":"Abcam, ab250896, Anti-PGK1 + PGK2 antibody [EPR14908] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal PGK1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR14908,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab250896 is the carrier-free version of\u003cbr\u003e\nab186742\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPhosphoglycerate kinase 1 (PGK1) and phosphoglycerate kinase 2 (PGK2) are enzymes involved in glycolytic processes. PGK1 also known as PGKA has a molecular mass of approximately 45 kDa and is widely expressed in most tissues especially in those requiring high energy. PGK2 alternatively known as testis-specific PGK has a similar molecular mass of about 45 kDa and is mainly expressed in the testis. Both enzymes ensure the important step of catalyzing the transfer of a phosphate group from 13-bisphosphoglycerate to ADP forming 3-phosphoglycerate and ATP.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPhosphoglycerate kinase 1 and 2 are important for cellular energy production and metabolic homeostasis. PGK1 plays a role in maintaining general cellular energy needs especially in environments with fluctuating oxygen levels. PGK2 as a testis-specific isoform supports spermatogenesis by facilitating glycolysis important for sperm motility. Both enzymes do not form part of a larger enzymatic complex functioning as standalone entities in their respective pathways.\u003cbr\u003e\nPathways\u003cbr\u003e\nPhosphoglycerate kinases participate in the glycolytic pathway which is essential for glucose catabolism and ATP generation. Within glycolysis PGK1 and PGK2 work with other glycolytic enzymes such as hexokinase and pyruvate kinase. Both enzymes help balance cellular redox states through their action in redox and energy production pathways intimately linked to other metabolic states such as those found in oxidative phosphorylation.\u003cbr\u003e\nOveractivity of PGK1 has associations with cancer where altered glycolytic activity supports rapid tumor growth. Its partner proteins like HIF-1α help mediate these changes in cancer metabolism. Additionally PGK2 deficiency can result in male infertility due to its indispensable role in sperm energy metabolism. There is no prominent disease linked partner for PGK2 outside its glycolytic function within testis tissue.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851887071401,"sku":"ab250896","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab250896","provider":"Iright","version":"1.0","type":"link"}