{"product_id":"abcam-ab252887","title":"Abcam, ab252887, Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nLysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) ab252887 provides a simple way to quantify GAA activity. Readout on any fluorometric (Ex\/Em 368\/460 nm) plate reader. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Tissue Homogenate, Other biological fluids, Cell Lysate,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:= 0.05 µU,\u003cbr\u003e\nAssay Platform:Microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nLysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) ab252887 provides a simple way to monitor GAA activity in a wide variety of biological samples. In this kit, GAA cleaves a synthetic specific substrate releasing a fluorophore, which can be easily quantified (Ex\/Em= 368\/460 nm). The assay is specific and sensitive, it can detect as low as 0.05 μU of GAA activity.\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K187 Lysosomal a-Glucosidase (GAA) Activity Assay Kit (Fluorometric). K187-100 is the same size as the 100 test size of ab252887.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlpha-glucosidase also known as alpha-14-glucosidase is an enzyme with a mass of approximately 105 kDa. It plays an important role in carbohydrate metabolism by catalyzing the hydrolysis of alpha-glycosidic bonds releasing glucose from the non-reducing end of glycogen. This enzyme is mostly present in the brush border of the small intestine where it facilitates the final step of complex carbohydrate digestion. Its activity is essential for the conversion of disaccharides and oligosaccharides into glucose and tools like alpha-glucosidase assays help in quantifying this activity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAlpha-glucosidase activity is important for maintaining normal glucose levels in the blood. It forms part of a larger enzyme complex that operates along the intestinal microvilli. Within the complex it collaborates with other hydrolases to optimize disaccharide breakdown. This role ensures the efficient transformation of ingested carbohydrates into absorbable monosaccharides which is an important cellular energy source.\u003cbr\u003e\nPathways\u003cbr\u003e\nAlpha-glucosidase participates in the digestive process pathways notably the starch and sucrose metabolism pathway. It acts downstream of amylase enzymes that break down polysaccharides. This enzyme works closely with other glucosidases and maltase-glucoamylase within these pathways to terminate the carbohydrate digestion. Proteins like sucrase-isomaltase also interact in these pathways enhancing carbohydrate assimilation.\u003cbr\u003e\nAlpha-glucosidase is associated mainly with Pompe disease and diabetes mellitus. Pompe disease results from a defect in the related protein acid alpha-glucosidase (GAA) leading to glycogen accumulation in lysosomes. In diabetes inhibiting intestinal alpha-glucosidase reduces postprandial blood glucose levels making it a target for therapeutic drugs. Understanding these connections aids in developing treatments that modulate enzyme activity to manage carbohydrate metabolism disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843648344233,"sku":"ab252887","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab252887","provider":"Iright","version":"1.0","type":"link"}