{"product_id":"abcam-ab253867","title":"Abcam, ab253867, Human AKT1 (phospho S473) + AKT3 (phospho S472) Antibody Pair - BSA and Azide free","description":"\u003cp\u003eSize: 10 x 96Tests\u003cbr\u003e\nHuman AKT1 (phospho S473) + AKT3 (phospho S472) Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human AKT1 (phospho S473) + AKT3 (phospho S472).\u003cbr\u003e\nKey facts\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:ELISA set,\u003cbr\u003e\nAssay Platform:Reagents\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman AKT1 (phospho S473) + AKT3 (phospho S472) is a matched pair of unconjugated\u003cbr\u003e\nrecombinant rabbit monoclonal\u003cbr\u003e\ncapture and detection antibodies used to quantify Human AKT1 (phospho S473) + AKT3 (phospho S472) in sandwich ELISAs and many other pair-based applications.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nUse our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with \u0026lt;1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nThe pair can be used in variety of assays and platforms including but not limited to:\u003cbr\u003e\nSandwich ELISA\u003cbr\u003e\nFRET\/TR-FRET\/HTRF\u003cbr\u003e\nMeso Scale Discovery\u003cbr\u003e\nBead-based assays\u003cbr\u003e\nAlphaLISA\u003cbr\u003e\n\/AlphaScreen\u003cbr\u003e\nDELFIA\u003cbr\u003e\nimmunoassays\u003cbr\u003e\nand Single Molecule Counting (SMC™) immunoassays\u003cbr\u003e\nMultiplex\u003cbr\u003e\nOur antibody pairs are supplied in a carrier-free format that is conjugation-ready:\u003cbr\u003e\nBuffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.\u003cbr\u003e\nConcentration of ~1 mg\/ml as measured by the protein A280 method.\u003cbr\u003e\nWe can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.\u003cbr\u003e\nPairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.\u003cbr\u003e\nPlease note:\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.\u003cbr\u003e\nThe range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.\u003cbr\u003e\nWe guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.\u003cbr\u003e\nAntibody properties:\u003cbr\u003e\nCapture antibody:\u003cbr\u003e\nrecombinant rabbit monoclonal (unconjugated) – 100 µg\u003cbr\u003e\nDetector antibody:\u003cbr\u003e\nrecombinant rabbit monoclonal (unconjugated) - 100 µg\u003cbr\u003e\nConcentration\u003cbr\u003e\n: ~1 mg\/ml\u003cbr\u003e\nStorage buffer:\u003cbr\u003e\n100% PBS\u003cbr\u003e\nLiquid\u003cbr\u003e\nIsotype:\u003cbr\u003e\nRecombinant monoclonal antibodies offer several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free production\u003cbr\u003e\nMeso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.\u003cbr\u003e\nAlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.\u003cbr\u003e\nSimoa is a registered trademark of Quanterix, Inc.\u003cbr\u003e\nSMC is a registered trademark of Merck KGaA, Darmstadt, Germany.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAKT proteins also known as protein kinase B (PKB) play a significant role in cell signaling. AKT1 and AKT3 with AKT1 also being known as RAC-alpha serine\/threonine-protein kinase share a highly conserved structure and function. These proteins are typically around 56 kDa in size. They are ubiquitously expressed in various tissues with AKT3 having higher expression levels in the brain. The phosphorylation of AKT at Ser473 is an important process for its activation making 'AKT (phospho Ser473)' a critical form for functional studies.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAKT proteins influence multiple cellular processes including growth survival and metabolism. AKT1 and AKT3 do not function in isolation but are part of the PI3K\/AKT\/mTOR signaling complex. The activation of these proteins regulates downstream targets that facilitate various critical cellular functions. This includes cell cycle progression and inhibition of apoptotic processes. The PI3K\/AKT pathway drives many responses necessary for cellular homeostasis with other proteins like mTOR being involved.\u003cbr\u003e\nPathways\u003cbr\u003e\nAKT proteins integrate signals from the PI3K\/AKT\/mTOR pathway. This pathway is essential in transmitting signals related to cell survival and proliferation. It closely interacts with the insulin signaling pathway where AKT proteins play key roles in glucose metabolism and glycogen synthesis. AKT1 and AKT3 act in conjunction with proteins like PI3K and mTOR bridging growth factor signals to functional cell responses.\u003cbr\u003e\nAKT proteins associate with cancer development and progression owing to their role in cell growth and survival. Malfunction or mutation leading to hyperactivation of AKT signaling contributes to uncontrolled cell proliferation a hallmark of cancer. These proteins also impact neurological disorders with links to conditions like schizophrenia. The involvement of AKT3 especially in the brain connects it with neurodevelopmental anomalies. AKT interacts with proteins such as PTEN in disease contexts influencing pathways related to tumor suppression and cellular regression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843609546921,"sku":"ab253867","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab253867","provider":"Iright","version":"1.0","type":"link"}